IgD Monoclonal Antibody (11-26c (11-26)), FITC

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IgD Monoclonal Antibody (11-26c (11-26)), FITC

货号:11-5993-82,11-5993-85

规格:100μg,500μg

价格:1545,2271

产品类型:流式抗体

品牌:Thermo Fisher

物种:小鼠

宿主:大鼠

抗体亚型:IgG2a, kappa

荧光染料:FITC

抗体类型 单抗同型对照:Rat IgG2a kappa Isotype Control (eBR2a), FITC
浓度:0.5 mg/mL用法:0.125 μg/test (Flow);
产品详细信息Description: The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells.Applications Reported: The 11-26c antibody has been reported for use in flow cytometric analysis to detect the surface IgD on mouse B cells.Applications Tested: This 11-26c (11-26) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.
数据
IgD Antibody (11-5993-82) in FlowStaining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.06 µg of Rat IgG2a K Isotype Control FITC (Product # 11-4321-42) (left) or 0.06 µg of Anti-Mouse IgD FITC (right). Cells in the lymphocyte gate were used for analysis.
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参考文献:
1.ImmunityOpposing signals from the Bcl6 transcription factor and the interleukin-2 receptor generate T helper 1 central and effector memory cells."11-5993 was used in Functional assays to study the generation of T helper 1 central and effector memory cells from opposing signals from the Bcl6 transcription factor and the interleukin-2 receptor."AuthorsPepper M,Pagán AJ,Igyártó BZ,Taylor JJ,Jenkins MK2.Nature immunologyThe nuclear receptor LXRα controls the functional specialization of splenic macrophages."11-5993 was used in Immunofluorescence to investigate the mechanisms that control the heterogeneity of tissue macrophages."AuthorsA-Gonzalez N,Guillen JA,Gallardo G,Diaz M,de la Rosa JV,Hernandez IH,Casanova-Acebes M,Lopez F,Tabraue C,Beceiro S,Hong C,Lara PC,Andujar M,Arai S,Miyazaki T,Li S,Corbi AL,Tontonoz P,Hidalgo A,Castrillo A3.Mucosal immunologyAntigen sampling by intestinal M cells is the principal pathway initiating mucosal IgA production to commensal enteric bacteria."11-5993 was used in Immunofluorescence to demonstrate that M-cell-mediated sampling of commensal bacteria is a required initial step for efficient induction of intestinal SIgA."AuthorsRios D,Wood MB,Li J,Chassaing B,Gewirtz AT,Williams IR4.GutEctopic lymphoid tissue alters the chemokine gradient, increases lymphocyte retention and exacerbates murine ileitis."11-5993 was used in Immunofluorescence to study the contribution of the CCR7/CCL19/CCL21 chemokine axis during the development of tertiary lymphoid tissue and its role in disease."AuthorsMcNamee EN,Masterson JC,Jedlicka P,Collins CB,Williams IR,Rivera-Nieves J5.Nature communicationsDifferential cell-intrinsic regulations of germinal center B and T cells by miR-146a and miR-146b."Published figure using IgD monoclonal antibody (Product # 11-5993-82) in Immunofluorescence"
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