CD25 Monoclonal Antibody (BC96), APC, eBioscience™

货号：17-0259-41,17-0259-42

规格：25 Tests,100 Tests

价格：1518,3035

产品类型：流式抗体

品牌：eBioscience

抗原：CD7

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：BC96

荧光染料：APC

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test（Flow）

产品详细信息Description: The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ralpha). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation.<br>Applications Reported: The BC96 antibody has been reported for use in flow cytometric analysis.Applications Tested: This BC96 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.仅用于科研。不用于诊断过程。

数据

CD25 Antibody (17-0259-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD4 PE (Product # 12-0049-42) and Mouse IgG1 kappa Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD25 APC (right). Cells in the lymphocyte gate were used for analysis.

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参考文献

1.Scientific reportsCombinations of isoform-targeted histone deacetylase inhibitors and bryostatin analogues display remarkable potency to activate latent HIV without global T-cell activation.AuthorsAlbert BJ,Niu A,Ramani R,Marshall GR,Wender PA,Williams RM,Ratner L,Barnes AB,Kyei GB2.Immunity and ageing : I and ADepressive symptoms post hip fracture in older adults are associated with phenotypic and functional alterations in T cells."17-0259 was used in Flow cytometry/Cell sorting to investigate the synergistic effects of hip fracture physical trauma and psychological depressive symptoms on the aged immune system."AuthorsDuggal NA,Upton J,Phillips AC,Hampson P,Lord JM3.Journal of immunology researchThe Genetic Polymorphisms of NLRP3 Inflammasome Associated with T Helper Cells in Patients with Multiple Myeloma.AuthorsZhao X,Hua M,Yan S,Yu J,Han F,Zhong C,Wang R,Zhang C,Hou M,Ma D4.Biology of reproductionExpression and function of PDCD1 at the human maternal-fetal interface."17-0259 was used in Flow cytometry/Cell sorting to examine whether human maternal T cells express PDCD1."AuthorsTaglauer ES,Trikhacheva AS,Slusser JG,Petroff MG5.Journal of immunology (Baltimore, Md. : 1950)Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells."17-0259 was used in Flow cytometry/Cell sorting to study the effects of Foxp3 and IL-2 on FCRL3-/+ nTreg cell subsets."AuthorsNagata S,Ise T,Pastan I

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