CD25 Monoclonal Antibody (BC96), PE-Cyanine5, eBioscience™

货号：15-0259-42

规格：100 Tests

价格：3035

产品类型：流式抗体

品牌：eBioscience

抗原：CD25

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：BC96

荧光染料：PE-Cy5

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine5, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (0.25 µg)/test（Flow）

产品详细信息Description: The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ralpha). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation.<br>Applications Reported: This BC96 antibody has been reported for use in flow cytometric analysis.Applications Tested: This BC96 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.仅用于科研。不用于诊断过程。

数据

CD25 Antibody (15-0259-42) in FlowStaining of 3-day PHA activated normal human peripheral blood mononuclear cells with Mouse IgG1 kappa Isotype Control PE-Cyanine5 (Product # 15-4714-81) (open histogram) or Anti-Human CD25 PE-Cyanine5 (filled histogram).Total cells were used for analysis. CD25 Antibody (15-0259-42)Frontiers in pharmacology 2020 -FIGURE 5 Effects of disruption of PD-1 on cytotoxicity, cytokines production, and phenotype of GPC3-CAR T cells in vitro . (A) Cytotoxic activities of UTD and GPC3-CAR T cells with intact or deficient PD-1 against GPC3-positive (PLC/PRF/5 and SK-HEP-1/GPC3) and GPC3-negative (SK-HEP-1) HCC cells at an effector:target ratio of 1:1. (B,C) The production of IL-2 (B) and IFN-gamma (C) by the UTD and GPC3-CAR T cells with intact or deficient PD-1 cocultured with GPC3-positive (PLC/PRF/5 and SK-HEP-1/GPC3) or GPC3-negative (SK-HEP-1) HCC cells at an effector:target ratio of 1:1. (D) CD4 and CD8 subsets constitution of wild-type and PD-1-deficient GPC3-CAR T cells. The expressions of CD4 and CD8 on CAR T cells were measured by flow cytometry. (E) The expressions of early (CD69), and intermediate or late (CD25) activation markers on cell surface of wild-type and PD-1-deficient GPC3-CAR T cells. The expressions of CD25 and CD69 were determined by flow cytometry. Data shown were mean +- SD from triplicates. Bars, SD. * P < 0.05, ** P < 0.01, and *** P < 0.001 by one way ANOVA with Turkey post hoc test

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参考文献

1.MedicineFunction of Treg Cells Decreased in Patients With Systemic Lupus Erythematosus Due To the Effect of Prolactin."15-0259 was used in Flow cytometry/Cell sorting to investigate the role of prolactin in Treg-mediated immune response, showing that it decreased Treg function in patients with systemic Lupus Erythematosus."AuthorsLegorreta-Haquet MV,Chávez-Rueda K,Chávez-Sánchez L,Cervera-Castillo H,Zenteno-Galindo E,Barile-Fabris L,Burgos-Vargas R,Álvarez-Hernández E,Blanco-Favela F2.Journal of immunology (Baltimore, Md. : 1950)Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells."Published figure using CD25 monoclonal antibody (Product # 15-0259-42) in Flow Cytometry"3.Experimental and therapeutic medicineEquilibrium of Treg/Th17 cells of peripheral blood in syphilitic patients with sero-resistance."Published figure using CD25 monoclonal antibody (Product # 15-0259-42) in Flow Cytometry"4.Journal of immunology (Baltimore, Md. : 1950)Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells."Published figure using CD25 monoclonal antibody (Product # 15-0259-42) in Flow Cytometry"5.Journal of immunology (Baltimore, Md. : 1950)CpG and non-CpG oligodeoxynucleotides directly costimulate mouse and human CD4+ T cells through a TLR9- and MyD88-independent mechanism."15-0259 was used in Flow cytometry/Cell sorting to study the ability of oligodeoxynucleotides to activate and polarise T cells."AuthorsLandrigan A,Wong MT,Utz PJ

技术参数

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