CD4 Monoclonal Antibody (RPA-T4), PE
货号：12-0049-41,12-0049-42
规格：25 Tests,100 Tests
价格：1011,2323
产品类型：流式抗体
品牌：Thermo Fisher
物种：人
宿主：小鼠
抗体亚型：IgG1, kappa
荧光染料：PE
| 抗体类型 | 单抗 | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE |
| 浓度： | 5 µL/Test | 用法： | 5 μL (0.5 μg)/test (Flow) |
| 产品详细信息Description: The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). RPA-T4 blocks HIV binding and mixed lymphocyte reaction. The RPA-T4 antibody recognizes a different epitope than the OKT4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.Applications Reported: The RPA-T4 antibody has been reported for use in flow cytometric analysis.Applications Tested: The FITC conjugated RPA-T4 is offered in 2 formats:- µg size: has been tested by flow cytometric analysis and can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.- test size: has been pre-titrated and tested by flow cytometric analysis and can be used at 5 µL (0.5 µg) per test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017]. |
| 数据 |
| CD4 Antibody (12-0049-41) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (red histogram) or Anti-Human CD4 PE (green histogram). |
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| 参考文献： |
| 1.Molecular and cellular biologyForced expression of cyclin-dependent kinase 6 confers resistance of pro-B acute lymphocytic leukemia to Gleevec treatment."12-0049 was used in Flow cytometry/Cell sorting to find that forced expression of Cdk6 promotes continued cell division and decreased Gleevec-induced apoptosis of leukaemic cells."AuthorsKuo TC,Chavarria-Smith JE,Huang D,Schlissel MS2.Proceedings of the National Academy of Sciences of the United States of AmericaRemarkably low affinity of CD4/peptide-major histocompatibility complex class II protein interactions."Published figure using CD4 monoclonal antibody (Product # 12-0049-42) in Flow Cytometry"3.The journal of gene medicineInhibition of HIV-1 infection by a unique short hairpin RNA to chemokine receptor 5 delivered into macrophages through hematopoietic progenitor cell transduction."12-0049 was used in Flow cytometry/Cell sorting to assess the activity of the short hairpin RNA through haematopoietic progenitor cell (HPC) transduction and differentiation into macrophages derived from foetal liver CD34+ HPCs."AuthorsLiang M,Kamata M,Chen KN,Pariente N,An DS,Chen IS4.Acta Cardiologica SinicaThe Effects of High Mobility Group Box-1 Protein on Peripheral Treg/Th17 Balance in Patients with Atherosclerosis."Published figure using CD4 monoclonal antibody (Product # 12-0049-42) in Flow Cytometry"5.CellRAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses."Published figure using CD4 monoclonal antibody (Product # 12-0049-42) in Flow Cytometry" |
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