FOXP3 Monoclonal Antibody (236A/E7), APC, eBioscience™

货号：17-4777-42,17-4777-41

规格：100 Tests,25 Tests

价格：3163,1474

产品类型：流式抗体

品牌：eBioscience

抗原：FOXP3

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：236A/E7

荧光染料：APC

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test（Flow）

产品详细信息Description: The 236A/E7 antibody reacts with human Foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol reveals staining of the CD4+CD25bright population.The epitope from 236A/E7 is different from that of PCH101 (cat. 72-5776). This antibody has also been shown to recognize rhesus macaque, sooty mangabey and cynomolgus macaque.Applications Reported: This 236A/E7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 236A/E7 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Buffer Set (cat. 00-5523) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.仅用于科研。不用于诊断过程。

数据

FOXP3 Antibody (17-4777-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD4 FITC (Product # 11-0049-42) (left) and Anti-Human CD25 PE (Product # 12-0259-80) (right), followed by intracellular staining with Anti-Human Foxp3 APC, using Foxp3/Transcription Factor Staining Buffers (Product # 00-5523-00).

推荐产品：

-Intracellular Fixation & Permeabilization Buffer Set（货号#88-8824）-Foxp3 / Transcription Factor Staining Buffer Set（货号#00-5523-00）-Flow Cytometry Staining Buffer（货号#00-4222-57/26）

参考文献

1.Frontiers in immunologyTGF-β Affects the Differentiation of Human GM-CSF+CD4+T Cells in an Activation- and Sodium-Dependent Manner."17-4777 was used in Flow cytometry/Cell sorting to study the environmental cues that promote differentiation of granulocyte-macrophage colony-stimulating factor producing T cells."AuthorsÉliás S,Schmidt A,Kannan V,Andersson J,Tegnér J2.Frontiers in immunologyPhosphoproteomics Reveals Regulatory T Cell-Mediated DEF6 Dephosphorylation That Affects Cytokine Expression in Human Conventional T Cells."17-4777 was used in Flow cytometry/Cell sorting to investigate Treg suppression mechanisms."AuthorsJoshi RN,Binai NA,Marabita F,Sui Z,Altman A,Heck AJR,Tegnér J,Schmidt A3. Frontiers in immunologyMonomeric Immunoglobulin A from Plasma Inhibits Human Th17 ResponsesIn VitroIndependent of FcαRI and DC-SIGN."17-4777 was used in Flow cytometry/Cell sorting to suggest the potential therapeutic utility of monomeric IgA in autoimmune and inflammatory diseases that implicate Th17 cells."AuthorsSaha C,Das M,Patil V,Stephen-Victor E,Sharma M,Wymann S,Jordi M,Vonarburg C,Kaveri SV,Bayry J4.Science translational medicineInducing CTLA-4-dependent immune regulation by selective CD28 blockade promotes regulatory T cells in organ transplantation."17-4777 was used in Immunohistochemistry to suggest that targeting costimulation blockade at CD28 favors CTLA-4-dependent immune regulation and promotes allograft survival."AuthorsPoirier N,Azimzadeh AM,Zhang T,Dilek N,Mary C,Nguyen B,Tillou X,Wu G,Reneaudin K,Hervouet J,Martinet B,Coulon F,Allain-Launay E,Karam G,Soulillou JP,Pierson RN,Blancho G,Vanhove B5.The Journal of infectious diseasesCD4(+) regulatory T cells in a cynomolgus macaque model of Mycobacterium tuberculosis infection.AuthorsGreen AM,Mattila JT,Bigbee CL,Bongers KS,Lin PL,Flynn JL

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