FluoReporter Cell-Surface Biotinylation Kit
货号：F20650
规格：1 kit
价格：5170
产品类型：蛋白标记
品牌：Thermo Fisher
| The FluoReporter® Cell-Surface Biotinylation Kit provides a convenient method to label proteins exposed on the cell surface including, but not limited to, membrane proteins. The kit supplies 5 vials of biotin-XX SSE, and each vial can be used for several 1-mL labeling reactions (containing 2.5 x 107cells/mL).Learn More About Protein LabelingWe offer a wide selection of Molecular Probes® protein and antibody labeling kits to fit your starting material and your experimental setup. SeeAntibody Labeling from A to Zor use ourLabeling Chemistry Selection Toolfor other choices. To learn more about our various kits readKits for Labeling Proteins and Nucleic Acids—Section 1.2in the Molecular Probes® Handbook. |
| 特点 |
| • Biotinylation is typically complete in under 1 hour• Optimized for 1 mL labeling reactions each containing 2.5 x 107cells/mL• Biotinylated proteins can be subsequently identified using western blot techniques and labeled avidin and streptavidin conjugates (find an anti-biotin antibody using ourSecondary Antibody Selector Tool) |
| 数据 |
| F20650Figure 4.5 Identification of cell-surface proteins in Jurkat cells labeled with the FluoReporter® Cell-Surface Biotinylation Kit (F20650). The labeled cells were fractionated by differential detergent extraction into membrane and cytosolic fractions. The proteins were then precipitated with acetone, separated on an SDS-polyacrylamide gel and blotted onto a PVDF membrane. Total proteins and biotinylated proteins were differentially stained using the Pro-Q® Western Blot Stain Kit #6 (P21862). Total proteins were detected with the SYPRO® Ruby protein blot stain component of the kit (left panel); biotinylated proteins were identified with alkaline phosphatase streptavidin in combination with the red-fluorescent substrate, DDAO phosphate (right panel). MW = protein molecular weight markers; Con A = biotinylated concanavalin A. |
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