CD69 Monoclonal Antibody (FN50), eFluor 450, eBioscience™

货号：48-0699-41,48-0699-42

规格：25tests,100tests

价格：1019,2490

产品类型：流式抗体

品牌：Thermo Fisher

抗原：CD69

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：FN50

荧光染料：eFluor 450

抗体类型:	流式抗体	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor 450, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.06 µg)/test (Flow）

产品详细信息Description:The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 is approximately 30 kDa and is expressed on the cell-surface as a disulfide-linked dimer. CD69 is rapidly upregulated upon activation and expressed on lymphocytes, monocytes and platelets.Applications Reported: This FN50 antibody has been reported for use in flow cytometric analysis.Applications Tested: This FN50 antibody has been pre-titrated and tested by flow cytometric analysis of PHA stimulated normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD69 Antibody (48-0699-41) in FlowStaining of PHA stimulated normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0037-42) and Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (left) or Anti-Human CD69 eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Journal of viral hepatitisEx vivo analysis of resident hepatic pro-inflammatory CD1d-reactive T cells and hepatocyte surface CD1d expression in hepatitis C."Published figure using CD69 monoclonal antibody (Product # 48-0699-42) in Flow Cytometry"2.Journal of leukocyte biologyThe tryptophan metabolite picolinic acid suppresses proliferation and metabolic activity of CD4+ T cells and inhibits c-Myc activation."48-0699 was used in Flow cytometry/Cell sorting to investigate the effect of picolinic acid on cell viability, c-MYC activation and CD4+ T cell effector functions."3.Scientific reportsCombinations of isoform-targeted histone deacetylase inhibitors and bryostatin analogues display remarkable potency to activate latent HIV without global T-cell activation."Published figure using CD69 monoclonal antibody (Product # 48-0699-42) in Flow Cytometry"

技术参数

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