CD45RA Monoclonal Antibody (HI100), Alexa Fluor 532, eBioscience™

货号：58-0458-42,58-0458-41

规格：100 Tests,25 Tests

价格：3236,1381

产品类型：流式抗体

品牌：eBioscience

抗原：CD45RA

物种：人

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：HI100

荧光染料：Alexa Fluor 532

类型:	一抗	同型对照：	IgG2b, kappa
浓度：	5 µL/Test	用法：	5 µL (0.5 µg)/test（Flow）

产品详细信息Description: The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ peripheral T lymphocytes, CD8+ peripheral T lymphocytes, and B cells. The CD45RA+ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.Applications Reported: This HI100 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HI100 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Alexa Fluor 532 is excited with the Green laser (532 nm) and emits at 561 nm. This cannot be used with the Yellow-Green laser (561 nm). We recommend using a 560/14 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser靶标信息CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing and these isoforms differ in their extracellular domains, however, they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage, with the exception of mature red cells and is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Diseases associated with CD45 dysfunction include severe combined immunodeficiency and Hepatitis C.仅用于科研。不用于诊断过程。

数据

CD45RA Antibody (58-0458-42) in FlowNormal human peripheral blood cells were stained with CD45RO Monoclonal Antibody, eFluor 450 (Product # 48-0457-42) and Mouse IgG2b kappa Isotype Control, Alexa Fluor 532 (Product # 58-4732-81) (left) or CD45RA Monoclonal Antibody, Alexa Fluor 532 (right). Cells in the lymphocyte gate were used for analysis.

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