CD279 (PD-1) Monoclonal Antibody (eBioJ105 (J105)), PE-eFluor 610
货号：61-2799-42
规格：100 Tests
价格：3585
产品类型：流式抗体
品牌：Thermo Fisher
物种：人
宿主：小鼠
抗体亚型：IgG1, kappa
荧光染料：PE-eFluor 610
| 抗体类型 | 单抗 | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-eFluor 610 |
| 浓度： | 5 µL/Test | 用法： | 5 μL (0.5 μg)/test (Flow) |
| 产品详细信息Description: The J105 monoclonal antibody reacts with the human PD-1 (programmed death-1), a 55 kDa member of the CD28 immunoglobulin superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes. Two novel members of the B7 family have been identified as the PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells.Costaining experiments suggest that eBioJ105 recognizes a different epitope than MIH4 (cat. 11-9969).Applications Reported: This eBioJ105 (J105) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioJ105 (J105) antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of theimmune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE |
| 数据 |
| CD279 (PD-1) Antibody (61-2799-42) in FlowStaining of unstimulated (blue histogram) or 3-day PHA-stimulated (purple histogram) normal human peripheral blood cells with Anti-Human CD279 (PD-1) PE-eFluor® 610. Viable cells, as determined by Fixable Viability Dye eFluor® 450, in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.Frontiers in immunologyCharacterizing the Role of Monocytes in T Cell Cancer Immunotherapy Using a 3D Microfluidic Model."Published figure using CD279 (PD-1) monoclonal antibody (Product # 61-2799-42) in Flow Cytometry"2.The Journal of experimental medicineThe adjuvant GLA-SE promotes human Tfh cell expansion and emergence of public TCRβ clonotypes."Published figure using CD279 (PD-1) monoclonal antibody (Product # 61-2799-42) in Flow Cytometry"3.PLoS pathogensExhaustion of Activated CD8 T Cells Predicts Disease Progression in Primary HIV-1 Infection."61-2799 was used in Flow cytometry/Cell sorting to identify CD8 T cell exhaustion markers which correlate with HIV-1 disease progression." |
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