Mouse IgG2b kappa Isotype Control (eBMG2b), PE,
货号：12-4732-42
规格：100 Tests
价格：1164
产品类型：小鼠同型对照
品牌：Thermo Fisher
物种：其它
宿主：小鼠
抗体亚型：IgG2b, kappa
荧光染料：PE
| 抗体类型 | 同型对照 | 同型对照： | |
| 浓度： | 5 µL/Test | 用法： | 5 µL (1 µg)/test (Flow)；Assay-Dependent(Ctrl) |
| 产品详细信息Description: The monoclonal mouse IgG2b, kappa is useful as an isotype control immunoglobulin.Applications Reported: This mouse IgG2b isotype control has been reported for use in flow cytometric analysis.Applications Tested: This Mouse IgG2b K Isotype Control is offered in 2 formats: - µg size: has been tested by flow cytometric analysis of normal human peripheral blood cells and can be used at the same concentration as the experimental antibody. - test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at test size: 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody. |
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| 参考文献： |
| 1.mAbsA novel llama antibody targeting Fn14 exhibits anti-metastatic activity in vivo."12-4732 was used in Flow cytometry/Cell sorting to describe various novel mouse-human cross-reactive llama-derived recombinant Fn14-specific antibodies harboring the human IgG1 Fc domain."AuthorsTrebing J,Lang I,Chopra M,Salzmann S,Moshir M,Silence K,Riedel SS,Siegmund D,Beilhack A,Otto C,Wajant H2.Cancer biology and therapyAmyloid precursor-like protein 2 suppresses irradiation-induced apoptosis in Ewing sarcoma cells and is elevated in immune-evasive Ewing sarcoma cells."12-4732 was used in Flow cytometry/Cell sorting to examine the contribution of amyloid precursor-like protein 2 to the survival of Ewing sarcoma cells."3.Scientific reportsPlatelets confound the measurement of extracellular miRNA in archived plasma."12-4732 was used in Flow cytometry/Cell sorting to show that a single freeze/thaw cycle of plasma dramatically increases the number of platelet-derived MPs, contaminates the extracellular miRNA pool, and profoundly affects the levels of miRNAs detected."4.Scientific reportsPlatelets confound the measurement of extracellular miRNA in archived plasma."12-4732 was used in Flow cytometry/Cell sorting to show that a single freeze/thaw cycle of plasma dramatically increases the number of platelet-derived MPs, contaminates the extracellular miRNA pool, and profoundly affects the levels of miRNAs detected."5.Cancer researchPharmacological Inhibition of the Histone Lysine Demethylase KDM1A Suppresses the Growth of Multiple Acute Myeloid Leukemia Subtypes."12-4732 was used in Flow cytometry/Cell sorting to investigate the consequences of inhibiting lysine-specific demethylase 1 in a panel of cell lines representing all acute myelogenous leukaemia subtypes." |
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