CD5 Monoclonal Antibody (53-7.3), PE, eBioscience™

货号：12-0051-81,12-0051-82,12-0051-83

规格：50μg,100μg,200μg

价格：822,1545,2892

产品类型：流式抗体

品牌：eBioscience

抗原：CD5

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：53-7.3

荧光染料：PE

抗体类型:	小鼠流式抗体	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience
浓度：	0.2mg/mL	用法：	0.125 µg/test (Flow)

Description:The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67 kDa protein expressed by a majority of thymocytes, mature T cells and a subset of B cells. The expression of CD5 by a small subset of B cells characterizes a developmentally and functionally distinct lineage of B cells called B-1 cells. CD5 is a counter-receptor for CD72 and plays a role in the T-B cell interaction.Applications Reported: The 53-7.3 antibody has been reported for use in flow cytometric analysis.<br>Applications Tested: The 53-7.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD5 is a 67 kDa human T-lymphocyte single-chain transmembrane glycoprotein. CD5 is present on all mature T-lymphocytes, on most of thymocytes and on many T-cell leukemias and lymphomas. CD5 also reacts with a subpopulation of activated B-cells and may act as a receptor in regulating T-cell proliferation. CD5 is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells. Diseases associated with CD5 dysfunction include thymus cancer and Richter's Syndrome.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据：

CD5 Antibody (12-0051-81) in FlowStaining of BALB/c splenocytes with Anti-Mouse CD3 eFluor® 450 (Product # 48-0032-82) and 0.06 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (left) or 0.06 µg of Anti-Mouse CD5 PE (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.OncogeneMinor cell-death defects but reduced tumor latency in mice lacking the BH3-only proteins Bad and Bmf."12-0051 was used in Flow cytometry/Cell sorting to show that Bad and Bmf co-regulate lymphocyte homeostasis and limit spontaneous transformation in mice."2.AutoimmunityA new site-directed transgenic rheumatoid factor mouse model demonstrates extrafollicular class switch and plasmablast formation."12-0051 was used in Flow cytometry/Cell sorting to show that switched auto-Ab-forming cells develop robustly outside germinal centers, further confirming the extrafollicular expression of AID."3.PLoS pathogensEBV latent membrane protein 1 activates Akt, NFkappaB, and Stat3 in B cell lymphomas."12-0051 was used in Flow cytometry/Cell sorting to suggest that Akt, NFkappaB, and Stat3 pathways may serve as effective targets in the treatment of EBV-associated B cell lymphomas."

技术参数

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