CD5 Monoclonal Antibody (53-7.3), Super Bright 436, eBioscience™
货号：62-0051-82
规格：100μg
价格：3150
产品类型：流式抗体
品牌：eBioscience
抗原：CD5
物种：小鼠
宿主：大鼠
抗体亚型：IgG2a, kappa
克隆号：53-7.3
荧光染料：Super Bright 436
抗体类型: | 小鼠流式抗体 | 同型对照： | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright 436, eBioscience |
浓度： | 0.2mg/mL | 用法： | 0.25 µg/test (Flow) |
Description:The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67 kDa protein expressed by a majority of thymocytes, mature T cells and a subset of B cells. The expression of CD5 by a small subset of B cells characterizes a developmentally and functionally distinct lineage of B cells called B-1 cells. CD5 is a counter-receptor for CD72 and plays a role in the T-B cell interaction.Applications Reported: This 53-7.3 antibody has been reported for use in flow cytometric analysis.<br>Applications Tested: This 53-7.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 436 nm; Laser: Violet LaserSuper Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.Target InformationCD5 is a 67 kDa human T-lymphocyte single-chain transmembrane glycoprotein. CD5 is present on all mature T-lymphocytes, on most of thymocytes and on many T-cell leukemias and lymphomas. CD5 also reacts with a subpopulation of activated B-cells and may act as a receptor in regulating T-cell proliferation. CD5 is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells. Diseases associated with CD5 dysfunction include thymus cancer and Richter's Syndrome.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
| 数据： |
| CD5 Antibody (62-0051-82) in FlowC57BL/6 mouse splenocytes were stained with CD45R (B220) Monoclonal Antibody, FITC (Product # 11-0452-82) and 0.125 µg of Rat IgG2a kappa Isotype Control, Super Bright 436 (Product # 62-4321-82) (left) or 0.125 µg of CD5 Monoclonal Antibody, Super Bright 436 (right). Cells in the lymphocyte gate were used for analysis. |
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