CD90.2 (Thy-1.2) Monoclonal Antibody (30-H12), PE,

货号：12-0903-81,12-0903-82,12-0903-83

规格：50 ug ,100 ug ,200 ug

价格：727,1340,1752

产品类型：流式抗体

品牌：eBioscience

抗原：CD90.2 (Thy-1.2)

物种：人

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：30-H12

荧光染料：PE

类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), PE
浓度：	0.2 mg/mL	用法：	0.125 µg/test(Flow)

产品详细信息Description: The 30-H12 monoclonal antibody reacts with mouse CD90.2, also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/J, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 30-H12. CD90 is involved in regulation of adhesion and signal transduction by T cells.Applications Reported: The 30-H12 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 30-H12 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD90.2 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90.2 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons in all species studied, highly expressed in connective tissue, various fibroblast and stromal cell lines and lastly, by tumor endothelial cell lines. In the mouse, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.

数据

CD90.2 (Thy-1.2) Antibody (12-0903-81) in FlowStaining of C57Bl/6 splenocytes with Anti-Mouse CD3e APC (Product # 17-0031-82) and 0.06 µg of Rat IgG2b kappa Isotype Control PE (Product # 12-4031-82) (left) or 0.06 µg of Anti-Mouse CD90.2 (Thy-1.2) PE (right). Cells in the lymphocyte gate were used for analysis. CD90.2 (Thy-1.2) Antibody (12-0903-81)Nature communications 2015 -Figure 1 AA combined with 2i enhances reprogramming efficiency. ( a ) Counts of Nanog-positive colonies on day 17 of MEF reprogramming. Error bars represent standard deviation of four replicates. AA, ascorbic acid; 2i, MAP kinase inhibitor (PD-0325901)+glycogen synthetase kinase inhibitor (CHIR-99021). ( b ) Same as above but for adult fibroblasts. Technical replicates are stacked. Rep1 and Rep2 indicate two different genetic backgrounds. ( c ) Inset--Scheme of experiment: JSS MEFs received doxycycline (dox) for OSKM induction from day 0 to day 17 and were treated with DMSO, AA, 2i or AA+2i for the days indicated (grey bars). Counts of Nanog-positive colonies for four replicates. * P <0.05 ** P <0.01 assessed by t -test. ( d ) Top panel--Scheme of experiment: Cells were sorted for SSEA1 and lack of Thy1 expression (Thy1 - SSEA1 + ) on day 9 after dox induction. Sorted cells were exposed to DMSO, AA, 2i or AA+2i until day 13 or 16 post dox induction. Bottom panel--Bar graphs show combined results from two biological replicates (A and B) for Nanog-positive colonies after 4 (left) or 7 (right) days of treatment. * P <0.05 by t -test. ( e ) Left panel--Bar graph representing GFP reporter expression (under the control of the endogenous Nanog promoter) in pre-iPSCs upon exposure to AA and/or 2i as compared with DMSO by flow cytometry. Right panel--Counts of Nanog-GFP-positive ESC-like colonies that represent the Nanog-GFP-positive cells in the left panel. Inset: Image of Nanog-GFP-

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参考文献

1. Cell death & diseaseRap1 deficiency-provoked paracrine dysfunction impairs immunosuppressive potency of mesenchymal stem cells in allograft rejection of heart transplantation.2. Frontiers in immunologyLow-Dose Radiotherapy Ameliorates Advanced Arthritis in hTNF-α tg Mice by Particularly Positively Impacting on Bone Metabolism.3. PloS oneMononuclear Phagocytes Are Dispensable for Cardiac Remodeling in Established Pressure-Overload Heart Failure.4. The Journal of experimental medicineHierarchy of Notch-Delta interactions promoting T cell lineage commitment and maturation.5. Cytometry. Part A : the journal of the International Society for Analytical CytologyCharacterization of fibroblasts responsible for cartilage destruction in arthritis.

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