| 产品详细信息Description: The eBioH35-17.2 monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. CD8 binds to MHC class I and plays a role in T cell development and activation of mature T cells.Applications Reported: This eBioH35-17.2 (H35-17.2) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections.Applications Tested: This eBioH35-17.2 (H35-17.2) antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. |
| 1.GutMyeloid cells are required for PD-1/PD-L1 checkpoint activation and the establishment of an immunosuppressive environment in pancreatic cancer."14008382 was used in immunohistochemistry to discuss the role of myeloid cells in pancreatic cancer pathogenesis"AuthorsZhang Y,Velez-Delgado A,Mathew E,Li D,Mendez FM,Flannagan K,Rhim AD,Simeone DM,Beatty GL,Pasca di Magliano M2.Viral immunologyIntraepithelial T-cell cytotoxicity, induced bronchus-associated lymphoid tissue, and proliferation of pneumocytes in experimental mouse models of influenza."14-0083 was used in Immunohistochemistry to examine the lungs of mice with experimental influenza virus."AuthorsSell S,Guest I,McKinstry KK,Strutt TM,Kohlmeier JE,Brincks E,Tighe M,Blackman MA,Woodland DL,Dutton RW,Swain SL3.Frontiers in immunologyAn Antigen-Presenting and Apoptosis-Inducing Polymer Microparticle Prolongs Alloskin Graft Survival by Selectively and Markedly Depleting Alloreactive CD8+T Cells."14-0083 was used in Immunohistochemistry to document the high potential of PLGA MP-based KaAPCs as a novel antigen-specific immunotherapy for allograft rejection and autoimmune disorder."AuthorsWang W,Shahzad KA,Li M,Zhang A,Zhang L,Xu T,Wan X,Shen C4.Mucosal immunologySoluble CD83 ameliorates experimental colitis in mice."14-0083 was used in Immunohistochemistry to investigate new strategies for treatment of IBD, showing that soluble CD83 ameliorates experimental colitis in mice."AuthorsEckhardt J,Kreiser S,Döbbeler M,Nicolette C,DeBenedette MA,Tcherepanova IY,Ostalecki C,Pommer AJ,Becker C,Günther C,Zinser E,Mak TW,Steinkasserer A,Lechmann M5.Cancer researchImmune response is an important aspect of the antitumor effect produced by a CD40L-encoding oncolytic adenovirus."14-0083 was used in Immunohistochemistry to test whether a transcriptionally targeted oncolytic adenovirus, which features a capsid modification and is armed with CD40L, can result in oncolytic antitumor activity and stimulate an immune response."AuthorsDiaconu I,Cerullo V,Hirvinen ML,Escutenaire S,Ugolini M,Pesonen SK,Bramante S,Parviainen S,Kanerva A,Loskog AS,Eliopoulos AG,Pesonen S,Hemminki A |
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