Endura™ ElectroCompetent Cells/Endura™电感受态细胞
货号：60242-1 ,60242-2
规格：12 rxns (DUO),24 rxns (DUO)
价格：2886,5039
产品类型：感受态细胞
品牌：Lucigen
| Lucigen’s Endura Competent Cells are noted for their ability to clone and maintain plasmid DNA sequences that are otherwise unstable, such as those with inverted repeats or sequences that are otherwise prone to undesired recombination events. Such unstable inserts are commonly found in retroviral DNA, and can prove exceptionally difficult to isolate and propagate in traditional cloning strains. Lucigen’s Endura Competent Cells are available in high efficiency chemically competent or electrocompetent versions to make difficult cloning work more reliable and efficient. Lucigen的Endura感受态细胞具有克隆和维持原本不稳定的质粒DNA序列的能力，例如具有反向重复序列或原本容易发生不良重组事件的序列。 这种不稳定的插入片段通常在逆转录病毒DNA中发现，并证明在传统的克隆菌株中很难分离和繁殖。 Lucigen的Endura感受态细胞具有高效的化学感受态或电感受态形式，使困难的克隆工作更加可靠和高效。 |
| 优点： |
| ▪Stabilize direct repeats and create lentiviral constructs；▪Generatelentiviral guide RNA libraries；▪Recommended inCRISPR GeCKO library protocols.▪稳定直接重复序列并创建慢病毒构建体；▪生成慢病毒指导RNA文库；▪在CRISPR GeCKO库操作中推荐。 |
| 数据： |
| Figure 1.Cost per reaction of chemically competent cells of clone-stabilizing strains from other suppliers.图 1.其他供应商的克隆稳定菌株的化学感受态细胞的每次反应成本。Figure 2.Transformation efficiencies of electrocompetent clone-stabilizing strains.图 2.电感受态克隆稳定菌株的转化效率。 |
| 相关产品： |
| ▪Endura™ Chemically Competent Cells（货号#60240/60241）▪ Recovery Medium（货号#80026-1） |
技术参数 产品优点 - Stabilize direct repeats and create lentiviral constructs
- Generate lentiviral guide RNA libraries
- Recommended in CRISPR GeCKO library protocols
- Choose electrocompetent or chemically competent cells
- Highest efficiency commercially available cells for lentiviral cloning: over 1 × 107 cfu/µg (chem) or 1 × 1010 cfu/µg (electro)
- 稳定直接重复序列并创建慢病毒构建体
- 生成慢病毒指南RNA库
- 在CRISPR GeCKO文库操作中推荐
- 选择电感受态或化学感受态细胞
- 商业上可获得的效率最高的慢病毒克隆细胞：超过1×10^7 cfu / µg（化学）或1×10^10 cfu / µg（电）
产品应用 - Clone repetitive sequences and lentiviral libraries
- 克隆重复序列和慢病毒文库
组成成分 Endura™ Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube) or DUOs (2 transformations per tube) as indicated. Endura Electrocompetent cells are provided in DUOs format only. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
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