CD8a Monoclonal Antibody (53-6.7), eFluor 660
货号：50-0081-80,50-0081-82
规格：25μg,100μg
价格：1052,1996
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG2a, kappa
荧光染料：eFluor 660
| 抗体类型 | 一抗 | 同型对照： | Rat IgG2a kappa Isotype Control (eBR2a), eFluor 660 |
| 浓度： | 0.2 mg/mL | 用法： | 0.125 μg/test (Flow)； |
| 产品详细信息Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.Applications Reported: This 53-6.7 antibody has been reported for use in flow cytometric analysis and immunohistology staining of frozen tissue sections.Applications Tested: This 53-6.7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells. |
| 数据 |
| CD8a Antibody (50-0081-80) in FlowStaining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.06 µg of Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) (left) or 0.06 µg of Anti-Mouse CD8a eFluor® 660 (right). Total viable cells were used for analysis. |
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| 参考文献： |
| 1.Cardiovascular researchGenetic deletion of chemokine receptor Ccr7 exacerbates atherogenesis in ApoE-deficient mice."Published figure using CD8a monoclonal antibody (Product # 50-0081-82) in Flow Cytometry"2.Scientific reportsType 2 Innate Lymphocytes Actuate Immunity Against Tumours and Limit Cancer Metastasis."Published figure using CD8a monoclonal antibody (Product # 50-0081-82) in Immunohistochemistry"3.Scientific reportsDiscovery of a Metastatic Immune Escape Mechanism Initiated by the Loss of Expression of the Tumour Biomarker Interleukin-33."Published figure using CD8a monoclonal antibody (Product # 50-0081-82) in Flow Cytometry"4.Proceedings of the National Academy of Sciences of the United States of AmericaCD31 signals confer immune privilege to the vascular endothelium."Published figure using CD8a monoclonal antibody (Product # 50-0081-82) in Flow Cytometry"5.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant SurgeonsImaging Tolerance Induction in the Classic Medawar Neonatal Mouse Model: Active Roles of Multiple F1-Donor Cell Types."Published figure using CD8a monoclonal antibody (Product # 50-0081-82) in Immunocytochemistry" |
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