CD8a Monoclonal Antibody (53-6.7), Biotin, Functional Grade
货号：36-0081-85
规格：500 µg
价格：3669
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG2a, kappa
荧光染料：Biotin
| 抗体类型 | 一抗 | 同型对照： | |
| 浓度： | 0.5 mg/mL | 用法： | 0.5 μg/test (Flow)； |
| 产品详细信息Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alphaalpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gammadelta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alphaalpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.Applications Reported: This 53-6.7 antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections.Applications Tested: This 53-6.7 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.05 ng/ug cytokine as determined by the LAL assay.Storage and handling: Use in a sterile environment.靶标信息CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells. |
| 数据 |
| CD8a Antibody (36-0081-85)Pain research & management 2019 -Figure 6 Effects of CB 2 R agonist treatment on T-lymphocyte infiltration into the LSC of LP-BM5-infected animals. After LP-BM5 infection, synthetic CB 2 R ligands were injected (5 mg/kg, i.p.) twice/wk starting at 5 or 6 wk (HU308) p.i. At 12 wk p.i., LSCs were dissected and infiltrating leukocytes were banded on a 30-70% Percoll cushion, collected, and labeled with Abs specific for CD45, CD4, and CD8 for analysis by flow cytometry. (a) Representative contour plots show percentages of CD4 + and CD8 + T-lymphocytes within uninfected (UI), infected (IF), and infected (IF + indicated CB 2 R ligand), agonist-treated LSC. (b) Frequency and number of CD4 + T-cells infiltrating into the LSC of animals belonging to the indicated treatment groups. (c) Frequency and number of CD8 + T-cells infiltrating into the LSC of animals belonging to the indicated treatment groups. Pooled data present absolute numbers (mean +- SE) of CD4 + and CD8 + T-cells from two independent experiments using 4-6 animals per group. * p < 0.05; *** p < 0.001. |
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| 参考文献： |
| 1.Cardiovascular researchGenetic deletion of chemokine receptor Ccr7 exacerbates atherogenesis in ApoE-deficient mice."Published figure using CD8a monoclonal antibody (Product # 36-0081-85) in Flow Cytometry"2.Scientific reportsType 2 Innate Lymphocytes Actuate Immunity Against Tumours and Limit Cancer Metastasis."Published figure using CD8a monoclonal antibody (Product # 36-0081-85) in Immunohistochemistry"3.Scientific reportsDiscovery of a Metastatic Immune Escape Mechanism Initiated by the Loss of Expression of the Tumour Biomarker Interleukin-33."Published figure using CD8a monoclonal antibody (Product # 36-0081-85) in Flow Cytometry"4.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant SurgeonsImaging Tolerance Induction in the Classic Medawar Neonatal Mouse Model: Active Roles of Multiple F1-Donor Cell Types."Published figure using CD8a monoclonal antibody (Product # 36-0081-85) in Immunocytochemistry"5.Nature communicationsOncofetal gene SALL4 reactivation by hepatitis B virus counteracts miR-200c in PD-L1-induced T cell exhaustion."Published figure using CD8a monoclonal antibody (Product # 36-0081-85) in Immunofluorescence" |
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