CD8a Monoclonal Antibody (53-6.7), PE-eFluor 610
货号：61-0081-80,61-0081-82
规格：25 μg,100 μg
价格：1267,2886
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG2a, kappa
荧光染料：PE-eFluor 610
| 抗体类型 | 一抗 | 同型对照： | Rat IgG2a kappa Isotype Control (eBR2a), PE-eFluor 610 |
| 浓度： | 0.2 mg/mL | 用法： | 0.5 μg/test (Flow)； |
| 产品详细信息Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.Applications Reported: This 53-6.7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 53-6.7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells. |
| 数据 |
| CD8a Antibody (61-0081-80) in FlowStaining of mouse splenocytes with Anti-Mouse CD3 PerCP-eFluor® 710 (Product # 46-0032-82) and 0.25 µg of Rat IgG2a K Isotype Control PE-eFluor® 610 (Product # 61-4321-82) (left) or 0.25 µg of Anti-Mouse CD8a PE-eFluor® 610 (right). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.Frontiers in neurologyIL-2/Anti-IL-2 Complex Attenuates Inflammation and BBB Disruption in Mice Subjected to Traumatic Brain Injury."Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry"2.Cardiovascular researchGenetic deletion of chemokine receptor Ccr7 exacerbates atherogenesis in ApoE-deficient mice."Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry"3.JCI insightIL-7-dependent STAT1 activation limits homeostatic CD4+ T cell expansion."Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry"4.Frontiers in physiologyMacrophage Depletion Protects against Cigarette Smoke-Induced Inflammatory Response in the Mouse Colon and Lung."Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry"5.Frontiers in immunologyA New Adjuvant MTOM MediatesMycobacterium tuberculosisSubunit Vaccine to Enhance Th1-Type T Cell Immune Responses and IL-2+T Cells."Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry" |
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