CD8a Monoclonal Antibody (53-6.7)
货号：14-0081-82,14-0081-85,14-0081-86
规格：100μg,500μg,1mg
价格：1022,2176,3658
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG2a, kappa
荧光染料：其它
| 抗体类型 | 一抗 | 同型对照： | |
| 浓度： | 0.5 mg/mL | 用法： | 0.25 μg/test (Flow)； |
| 产品详细信息Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.Applications Reported: This 53-6.7 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections. It has also been reported in T cell activation, isolation and depletion of CD8+ cells, and blocking of ligand binding. (Please use Functional Grade purified 53-6.7, cat. 16-0081, in functional assays.).Applications Tested: The 53-6.7 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells. |
| 数据 |
| CD8a Antibody (14-0081-82) in FlowStaining of C57Bl/6 splenocytes with 0.125 µg of Rat IgG2a Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.125 µg of Anti-Mouse CD8 Purified (filled histogram) followed by Anti-Rat IgG FITC (Product # 11-4811-85). Total viable cells were used for analysis. |
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| 参考文献： |
| 1.Cardiovascular researchGenetic deletion of chemokine receptor Ccr7 exacerbates atherogenesis in ApoE-deficient mice."Published figure using CD8a monoclonal antibody (Product # 14-0081-82) in Flow Cytometry"2.TheranosticsCD4+and CD8a+PET imaging predicts response to novel PD-1 checkpoint inhibitor: studies of Sym021 in syngeneic mouse cancer models."14-0081 was used in Immunohistochemistry to develop radio-tracers, for detection and assessment of whole body CD4+ and CD8a+ status, in order to predict responses of tumours to an immune checkpoint inhibitor."3.Communications biology90Y-NM600 targeted radionuclide therapy induces immunologic memory in syngeneic models of T-cell Non-Hodgkin's Lymphoma.4.Journal for immunotherapy of cancerEnanDIM - a novel family of L-nucleotide-protected TLR9 agonists for cancer immunotherapy."14-0081 was used in Immunohistochemistry to show that EnanDIM® comprise a novel family of TLR9 agonists that facilitate an efficacious activation of both innate and adaptive immunity."5.Molecular medicine (Cambridge, Mass.)Fimasartan reduces neointimal formation and inflammation after carotid arterial injury in apolipoprotein E knockout mice. |
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