CD27 Monoclonal Antibody (LG.7F9), PE, eBioscience™

货号：12-0271-83,12-0271-82,12-0271-81

规格：200 µg,100 µg,50 µg

价格：3200,1854,1164

产品类型：流式抗体

品牌：eBioscience

抗原：CD27

物种：人/小鼠/大鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：LG.7F9

荧光染料：PE

类型:	一抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™
浓度：	0.2 mg/mL	用法：	0.125 µg/test（Flow）

产品详细信息Description: The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. It has been reported that triggering CD27 plays an important role in the maturation of CD4+ and CD8+ effector cells. LG.7F9 cross-reacts with human and rat CD27.Applications Reported: The LG.7F9 antibody has been reported for use in flow cytometric analysis.Applications Tested: The LG.7F9 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.仅用于科研。不用于诊断过程。

数据

CD27 Antibody (12-0271-83) in FlowStaining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) eFluor® 450 (Product # 48-0452-82) and 0.06 µg of Armenian Hamster IgG Isotype Control PE (Product # 12-4888-81) (left) or 0.06 µg of Anti-CD27 PE (right). Total viable cells were used for analysis.

推荐产品：

-Intracellular Fixation & Permeabilization Buffer Set（货号#88-8824）-Foxp3 / Transcription Factor Staining Buffer Set（货号#00-5523-00）-Flow Cytometry Staining Buffer（货号#00-4222-57/26）

参考文献：

1. Nature communicationsIdentification and characterization of latency-associated peptide-expressing γδ T cells."12-0271 was used in Flow cytometry/Cell sorting to describe a subset of regulatory γδ T cells that express the latency-associated peptide, a membrane-bound TGF-β1."AuthorsRezende RM,da Cunha AP,Kuhn C,Rubino S,M'Hamdi H,Gabriely G,Vandeventer T,Liu S,Cialic R,Pinheiro-Rosa N,Oliveira RP,Gaublomme JT,Obholzer N,Kozubek J,Pochet N,Faria AMC,Weiner HLYear2015SpeciesMouseDilution1:100 2. PLoS neglected tropical diseasesSm29, but not Sm22.6 retains its ability to induce a protective immune response in mice previously exposed to a Schistosoma mansoni infection."12-0271 was used in Flow cytometry/Cell sorting to investigate the potential of Sm29 and Sm22.6 as promising antigens to compose a vaccine against schistosomiasis."AuthorsAlves CC,Araujo N,dos Santos VC,Couto FB,Assis NR,Morais SB,Oliveira SC,Fonseca CTYear2015SpeciesMouse 3. Journal of immunology (Baltimore, Md. : 1950)Plasmodium suppresses expansion of T cell responses to heterologous infections."12-0271 was used in Flow cytometry/Cell sorting to investigate the mechanisms by which Plasmodium suppresses the expansion of T cell responses to heterologous coinfections."AuthorsWhite CE,Villarino NF,Sloan SS,Ganusov VV,Schmidt NWYear2015SpeciesMouse 4. Journal of immunology (Baltimore, Md. : 1950)Viral MHC class I-like molecule allows evasion of NK cell effector responses in vivo."12-0271 was used in Flow cytometry/Cell sorting to show that recognition of the viral protein m157 by the inhibitory Ly49C receptor allows escape from natural killer cell control of viral replication."AuthorsPyzik M,Dumaine A,Dumaine AA,Charbonneau B,Fodil-Cornu N,Jonjic S,Vidal SMYear2014SpeciesMouse 5. PloS oneTumor necrosis factor induces tumor promoting and anti-tumoral effects on pancreatic cancer via TNFR1."12-0271 was used in Flow cytometry/Cell sorting to assess the mechanisms underlying the contribution of tumour necrosis factor to carcinogenesis."AuthorsChopra M,Lang I,Salzmann S,Pachel C,Kraus S,Bäuerlein CA,Brede C,Garrote AL,Mattenheimer K,Ritz M,Schwinn S,Graf C,Schäfer V,Frantz S,Einsele H,Wajant H,Beilhack A

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-27613.htm