CD44 Monoclonal Antibody (IM7), Alexa Fluor 532,

货号：58-0441-82

规格：100 µg

价格：3050

产品类型：流式抗体

品牌：eBioscience

抗原：CD44

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：IM7

荧光染料：Alexa Fluor 532

类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor 532
浓度：	0.2 mg/mL	用法：	0.5 µg/test(Flow)

产品详细信息Description: The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.Applications Reported: This IM7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This IM7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Alexa Fluor 532 is excited with the Green laser (532 nm) and emits at 561 nm. This cannot be used with the Yellow-Green laser (561 nm). We recommend using a 560/14 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser靶标信息CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyteactivation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.

数据

CD44 Antibody (58-0441-82) in FlowBALB/c mouse splenocytes were stained with CD3 Monoclonal Antibody, eFluor 450 (Product # 48-0032-82) and 0.25 µg of Rat IgG2b kappa Isotype Control, Alexa Fluor 532 (Product # 58-4031-80) (left) or 0.25 µg of CD44 Monoclonal Antibody, Alexa Fluor 532 (right). Cells in the lymphocyte gate were used for analysis.

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