CD25 Monoclonal Antibody (eBio7D4 (7D4)), Biotin, eBioscience™

货号：13-0252-82,13-0252-85,13-0252-86

规格：100 μg,500 μg,1 mg

价格：1215,2432,4051

产品类型：流式抗体

品牌：eBioscience

抗原：CD25

物种：小鼠

宿主：大鼠

抗体亚型：IgM

克隆号： eBio7D4 (7D4)

荧光染料：Biotin

抗体类型:	小鼠流式抗体	同型对照：	Rat IgM Isotype Control (eBRM), Biotin, eBioscience
浓度：	0.5 mg/mL	用法：	0.125 µg/test (Flow)

Description:The eBio7D4 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor a chain (IL-2Ra). CD25 is expressed by early progenitors of the T and B lineage, as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiation and activation/proliferation. The epitopes recognized by eBio3C7, PC61.5 and eBio7D4 are all different allowing for depletion with one antibody (typically PC61.5) followed by subsequent detection with another one. eBio7D4 has been reported to block IL-2 binding but can be overcome by additional IL-2. The monoclonal antibody PC61.5 (cat. 16-0251) is recommended for in vivo studies.Purity is > 95% IgG2a as determined by SDS-gel electrophoresis. Endotoxin: <0.5 EU/µgApplications Tested: This eBio7D4 (7D4) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据：

CD25 Antibody (13-0252-82) in FlowStaining of C57BL/6 splenocytes with Anti-Mouse CD4 APC (Product # 17-0042-82) and 0.06 µg of Rat IgM kappa Isotype Control Biotin (Product # 13-4341-81) (left) or 0.06 µg of Anti-Mouse CD25 Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

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参考文献：

1.PloS onePartial depletion of regulatory T cells does not influence the inflammation caused by high dose hemi-body irradiation."13-0252 was used in Flow cytometry/Cell sorting to investigate the effects of partial depletion of regulatory T cells alongside ionizing radiation on inflammation and tissue injury in mice."2.Journal of immunology (Baltimore, Md. : 1950)Donor B cells in transplants augment clonal expansion and survival of pathogenic CD4+ T cells that mediate autoimmune-like chronic graft-versus-host disease."13-0252 was used in Flow cytometry/Cell sorting to investigate the mechanisms whereby donor B cells augment chronic graft-versus-host disease (cGVHD) pathogenesis, showing that B cells augment clonal expansion and survival of pathogenic CD4+ T cells that mediate autoimmune-like cGVHD."3.Cell reportsDifferential Roles of IL-2 Signaling in Developing versus Mature Tregs."13-0252 was used in Flow cytometry/Cell sorting to study the differential roles of IL-2 signalling in developing versus mature regulatory T cells."4.Journal of immunology (Baltimore, Md. : 1950)Hepatic stellate cells function as regulatory bystanders."13-0252 was used in Magnetic cell separation to suggest that HSC can function as regulatory bystanders, and therefore, by promoting Tregs and suppressing Th17 differentiation, they might represent key players in the mechanism that drives liver-induced tolerance."

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