CD152 (CTLA-4) Monoclonal Antibody (UC10-4B9), Biotin, eBioscience™

货号：13-1522-82,13-1522-85

规格：100 µg,500 µg

价格：1340,2677

产品类型：流式抗体

品牌：eBioscience

抗原：CD152

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：UC10-4B9

荧光染料：Biotin

类型:	一抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), Biotin, eBioscience™
浓度：	0.5 mg/mL	用法：	1 µg/test（Flow）

产品详细信息Description: The UC10-4B9 monoclonal antibody reacts with mouse CD152, also known as the cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells at low level and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cells to an activated stateby inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in a large proportion of newly synthesized CTLA-4 in response to TCR signaling to be localized intracellularly.Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4.Applications Reported: The UC10-4B9 antibody has been reported for use in flow cytometric analysis.Applications Tested: The UC10-4B9 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed on mainly CD4+ T cells. It binds the same ligands as CD28 (CD80 and CD86 on B cells and dendritic cells), but with higher affinity than CD28. However, in contrast to CD28 which enhances cell function when bound at the same time as the T cell receptor, CTLA4 inhbits the T cell and prevents it from functioning. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function.仅用于科研。不用于诊断过程。

数据

CD152 (CTLA-4) Antibody (13-1522-85)British journal of pharmacology 2018 -Published figure using CD152 (CTLA-4) monoclonal antibody (Product # 13-1522-85)

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参考文献：

1.PloS oneParicalcitol reduces peritoneal fibrosis in mice through the activation of regulatory T cells and reduction in IL-17 production."13-1522 was used in Flow cytometry/Cell sorting to show the activation of immunological regulatory mechanisms by vitamin D receptor signalling could prevent or reduce fibrosis."AuthorsGonzález-Mateo GT,Fernández-Míllara V,Bellón T,Liappas G,Ruiz-Ortega M,López-Cabrera M,Selgas R,Aroeira LS2.Nature communicationsIntratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity."Published figure using CD152 (CTLA-4) monoclonal antibody (Product # 13-1522-82) in Flow Cytometry"3.Nature communicationsInterdependent IL-7 and IFN-γ signalling in T-cell controls tumour eradication by combined α-CTLA-4+α-PD-1 therapy."Published figure using CD152 (CTLA-4) monoclonal antibody (Product # 13-1522-82) in Flow Cytometry"4.PloS oneFavorable alteration of tumor microenvironment by immunomodulatory cytokines for efficient T-cell therapy in solid tumors."Published figure using CD152 (CTLA-4) monoclonal antibody (Product # 13-1522-82) in Flow Cytometry"5.PloS oneParicalcitol reduces peritoneal fibrosis in mice through the activation of regulatory T cells and reduction in IL-17 production."13-1522 was used in Flow cytometry/Cell sorting to show the activation of immunological regulatory mechanisms by vitamin D receptor signalling could prevent or reduce fibrosis."AuthorsGonzález-Mateo GT,Fernández-Míllara V,Bellón T,Liappas G,Ruiz-Ortega M,López-Cabrera M,Selgas R,Aroeira LS

技术参数

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