CD152 (CTLA-4) Monoclonal Antibody (UC10-4B9), eBioscience™

货号：14-1522-82,14-1522-81, 14-1522-37,14-1522-95

规格：100 µg,50 µg,2 mg,10 mg

价格：1514,0,11562,28906

产品类型：流式抗体

品牌：eBioscience

抗原：CD152

物种：人/小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：UC10-4B9

荧光染料：Purified

类型:	一抗	同型对照：	IgG
浓度：	0.5 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The UC10-4B9 monoclonal antibody reacts with mouse CD152, also known as the cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells at low level and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cells to an activated stateby inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in a large proportion of newly synthesized CTLA-4 in response to TCR signaling to be localized intracellularly.Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4.Applications Reported: The UC10-4B9 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. It has also been reported in in vitro functional studies. (Please use Functional Grade purified UC10-4B9, cat. 16-1522, in functional assays.).Applications Tested: The UC10-4B9 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed on mainly CD4+ T cells. It binds the same ligands as CD28 (CD80 and CD86 on B cells and dendritic cells), but with higher affinity than CD28. However, in contrast to CD28 which enhances cell function when bound at the same time as the T cell receptor, CTLA4 inhbits the T cell and prevents it from functioning. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function.仅用于科研。不用于诊断过程。

数据

CD152 (CTLA-4) Antibody (14-1522-82)PloS one 2016 -Fig 6 Expression of anergy markers on CD8+ TILs are downregulated following IFN-alpha2, IFN-gamma and IL-2 treatments. Mice bearing subcutaneous B16.OVA tumors were injected with 2x10 6 CD8a + enriched OT-I lymphocytes into peritoneal cavity and beginning on the same day, tumors were injected with either PBS or recombinant cytokine in PBS or left non-injected (n = 5). Proportion of CD3 + CD8 + TILs expressing surface anergy markers (a) CTLA-4 and (b) PD-1 was analyzed by flow cytometry on day 14 post-transfer. Data presented as mean +- SEM. *P <= 0.05, **P<= 0.01, ***P<= 0.001, ****P<= 0.0001 by one-way ANOVA followed by Tukey's post-hoc test.

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参考文献：

1. Nature medicineAn asthma-associated IL4R variant exacerbates airway inflammation by promoting conversion of regulatory T cells to TH17-like cells."14-1522 was used in Flow cytometry/Cell sorting to identify a previously unknown mechanism for the development of TH17 cell inflammation in genetically prone individuals."AuthorsMassoud AH,Charbonnier LM,Lopez D,Pellegrini M,Phipatanakul W,Chatila TAYear2016SpeciesMouseDilution1:200 2. Clinical & translational immunologyConditions for the generation of cytotoxic CD4(+) Th cells that enhance CD8(+) CTL-mediated tumor regression."14-1522 was used in Flow cytometry/Cell sorting to provide valuable data for further research into in vitro expansion of CD4(+) Th1-like cells, with potential applications to cancer treatment involving ACT."AuthorsLi K,Baird M,Yang J,Jackson C,Ronchese F,Young SYear2016SpeciesMouse 3. PloS oneNfatc2 and Tob1 have non-overlapping function in T cell negative regulation and tumorigenesis."14-1522 was used in Flow cytometry/Cell sorting to study how Nfatc2 and Tob1 are non-redundant regulators of lymphocyte homeostasis."AuthorsMay SL,Zhou Q,Lewellen M,Carter CM,Coffey D,Highfill SL,Bucher CM,Matise I,Morse HC,O'Sullivan MG,Schutten M,Johnson C,Bellgrau D,Blazar BR,Modiano JFYear2015SpeciesMouse 4. Journal of immunology (Baltimore, Md. : 1950)Pak2 Links TCR Signaling Strength to the Development of Regulatory T Cells and Maintains Peripheral Tolerance."14-1522 was used in Flow cytometry/Cell sorting to show a novel role for the actin cytoskeletal remodeling protein, p21-activated kinase 2, in Treg development and homeostasis."AuthorsO'Hagan KL,Choi J,Pryshchep O,Chernoff J,Phee HYear2015SpeciesMouseDilution1:200 5. Journal of immunology (Baltimore, Md. : 1950)Regulatory T cells require TCR signaling for their suppressive function."14-1522 was used in Flow cytometry/Cell sorting to reveal that T cell receptor-mediated PLCγ activation is required for Tregs to inhibit Tconv proliferation."AuthorsSchmidt AM,Lu W,Sindhava VJ,Huang Y,Burkhardt JK,Yang E,Riese MJ,Maltzman JS,Jordan MS,Kambayashi T

技术参数

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