CD137 Ligand (4-1BB Ligand) Monoclonal Antibody (TKS-1), PE,

货号：12-5901-81,12-5901-82,12-5901-83

规格：50 ug, 100 ug ,200 ug

价格：720.75,1440,2164.5

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD137 Ligand (4-1BB Ligand)

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：TKS-1

荧光染料：PE

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), PE
浓度：	0.2 mg/mL	用法：	1 µg/test(Flow)

产品详细信息Description: The TKS-1 monoclonal antibody reacts with mouse 4-1BB Ligand (4-1BBL), a type II transmembrane protein and a member of the tumor necrosis factor superfamily. 4-1BBL is expressed by activated antigen presenting cells including B cells and macrophages. Interaction of 4-1BBL with 4-1BB (CDw137) is reported to deliver a costimulatory signal important in the T-APC interaction.Applications Reported: The TKS-1 antibody has been reported for use in flow cytometric analysis.Applications Tested: The TKS-1 antibody has been tested by flow cytometric analysis of resting and 2-3 day (IgM+CD40)-activated mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD137 ligand (CD137L) is a type II membrane protein and part of the TNF superfamily. CD137L is a co-stimulatory molecule that is expressed on antigen presenting cells (DCs, monocytes/macrophages, B cells) and is upregulated upon activation. Its receptor, CD137/4-1BB, is found on a variety of cells, including inflamed endothelial cells, where its expression enhances extravasation of CD137L expressing monocytes. Activated T cells also express CD137, and engagement with CD137L enhances T cell proliferation, IL-2 secretion, survival, and cytotoxic activity. CD137L is also expressed on a majority of B-cell lymphomas, including mantle cell lymphoma, follicular lymphoma, and diffuse large B-cell lymphoma (but it is lacking on Hodgkin lymphoma and T-cell lymphoma). CD137L is also expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interactions.

数据

CD137 Ligand (4-1BB Ligand) Antibody (12-5901-81) in FlowStaining of 3-day unstimulated (left) and 3-day Anti-Mouse IgM and Anti-Mouse CD40-stimulated (right) mouse splenocytes with 0.5 µg of Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (blue histogram) or 0.5 µg of Anti-Mouse CD137 Ligand (4-1BB Ligand) PE (Product # 12-5901-82) (purple histogram). Total viable CD45R (B220)+ cells were used for analysis.

推荐产品

二抗荧光二抗免疫组化一抗标签抗体

参考文献

1. The Journal of experimental medicineGalectin-9 controls the therapeutic activity of 4-1BB-targeting antibodies.2. Journal of immunology (Baltimore, Md. : 1950)The 4-1BB costimulation augments the proliferation of CD4+CD25+ regulatory T cells.3. OncoimmunologyAngiotensin converting enzyme inhibitors and angiotensin II receptor antagonist attenuate tumor growth via polarization of neutrophils toward an antitumor phenotype.4. Journal of immunology (Baltimore, Md. : 1950)The 4-1BB costimulation augments the proliferation of CD4+CD25+ regulatory T cells.

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-27667.htm