CD25 Monoclonal Antibody (PC61.5), eBioscience™

货号：14-0251-81,14-0251-85,14-0251-86

规格：50 μg,500 μg,1 mg

价格：0,2374,3797

产品类型：流式抗体

品牌：eBioscience

抗原：CD25

物种：小鼠

宿主：大鼠

抗体亚型：其它

克隆号：PC61.5

荧光染料：其它

抗体类型:	小鼠流式抗体	同型对照：	IgG1
浓度：	0.5 mg/mL	用法：	0.5 µg/test (Flow)

Description:The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation.Applications Reported: The PC61.5 antibody has been reported for use in flow cytometric analysis.Applications Tested: The PC61.5 antibody has been tested by flow cytometric analysis of mouse splenocytes or Con A-stimulated splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据：

CD25 Antibody (14-0251-81) in FlowStaining of 3-day ConA activated C57Bl/6 splenocytes with 0.25 µg of Rat IgG1 Isotype Control Purified (Product # 14-4301-82) (open histogram) or 0.25 µg of Anti-Mouse CD25 Purified (filled histogram) followed by Anti-Rat IgG FITC (Product # 11-4811-85). Total viable cells were used for analysis.

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参考文献：

1.Journal of immunology (Baltimore, Md. : 1950)Regulation of asymmetric division and CD8+ T lymphocyte fate specification by protein kinase Cζ and protein kinase Cλ/ι."14-0251 was used in Immunocytochemistry-immunoflourescence to show that PKCζ and PKCλ/i regulate asymmetric localization of effector fate-associated factors during the first CD8+ T cell division in vivo."2.Journal of immunology (Baltimore, Md. : 1950)Regulation of asymmetric division and CD8+ T lymphocyte fate specification by protein kinase Cζ and protein kinase Cλ/ι."14-0251 was used in Immunocytochemistry-immunoflourescence to show that PKCζ and PKCλ/i regulate asymmetric localization of effector fate-associated factors during the first CD8+ T cell division in vivo."3.OncoimmunologyCD4+ and CD8+ T cells are both needed to induce paraneoplastic neurological disease in a mouse model."14-0251 was used in Flow cytometry/Cell sorting to provide further insight into the cellular mechanisms whereby a potent anti-tumor immunity triggers a cancer-associated autoimmune disease, and may therefore help to develop new therapeutic strategies against PND."4.EMBO molecular medicineTGF-β1 secreted by Tregs in lymph nodes promotes breast cancer malignancy via up-regulation of IL-17RB."Published figure using CD25 monoclonal antibody (Product # 14-0251-86) in Neutralization"

技术参数

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