CD21 Monoclonal Antibody (HB5), PerCP-eFluor 710, eBioscience™

货号：46-0219-42

规格：100 Tests

价格：3330

产品类型：流式抗体

品牌：eBioscience

抗原：CD21

物种：人

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：HB5

荧光染料：PerCP-eFluor™ 710

抗体类型:	人CD21细胞流式抗体	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), PerCP-eFluor 710, eBioscience™
浓度：	5 μL/Test	用法：	5μL（0.125 µg）/test (Flow)

Description:The HB5 monoclonal antibody reacts with human CD21, an approximately 145 kDa type I transmembrane protein. CD21 is expressed by mature B cells, folliculate dendritic cells and a subset of epithelial cells and is a receptor for complement component C3d and Epstein-Barr virus (EBV). CD21, in association with CD19 and CD81, participates in the multimolecular complex with BCR and is involved in B cell activation.Applications Reported: This HB5 antibody has been reported for usein flow cytometric analysis.Applications Tested: This HB5 antibody has been pre-titrated and tested by flow cyometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD21 (complement receptor 2, CR2, C3D receptor, EBV receptor) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to follicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands. CD21 functions as receptor for C3d, C3dg and iC3b complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation. Genetic variations in the CD21 gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9). Alternatively, spliced transcript variants encoding different isoforms of CD21 have been found.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据：

CD21 Antibody (46-0219-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0037-42) and Mouse IgG2a K Isotype Control PerCP-eFluor® 710 (Product # 46-4724-82) (left) or Anti-Human CD21 PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Journal of immunology (Baltimore, Md. : 1950)Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion."Published figure using CD21 monoclonal antibody (Product # 46-0219-42) in Flow Cytometry"2.European journal of immunologyLong-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers."Published figure using CD21 monoclonal antibody (Product # 46-0219-42) in Flow Cytometry"

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