CD279 (PD-1) Monoclonal Antibody (RMP1-30), Super Bright 436
货号：62-9981-82
规格：100μg
价格：2948
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG2b, kappa
荧光染料：Super Bright 436
| 抗体类型 | 一抗 | 同型对照： | Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright 436 |
| 浓度： | 0.2 mg/mL | 用法： | 1 μg/test (Flow)； |
| 产品详细信息Description: The RMP1-30 antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligandsand their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. RMP1-30 does not block the binding of either B7-H1-Ig or B7-DC-Ig to PD-1 transfectants.Applications Reported: This RMP1-30 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RMP1-30 antibody has been tested by flow cytometric analysis of activated mouse splenocytes. This may be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Excitation: 405 nm; Emission: 436 nm; Laser: Violet LaserSuper Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.靶标信息Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of theimmune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE. |
| 数据 |
| CD279 (PD-1) Antibody (62-9981-82) in FlowBALB/c mouse splenocytes were unstimulated (left) or stimulated with CD3e and CD28 Monoclonal Antibodies, Functional Grade (Product # 16-0031-85andProduct # 16-0281-85) (right). Cells were then stained with 0.5 µg of Rat IgG2b kappa Isotype Control, Super Bright 436 (Product # 62-4031-82) (blue histogram) or CD279 Monoclonal Antibody, Super Bright 436 (purple histogram).Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50). |
| 推荐产品： |
| 二抗荧光二抗免疫组化一抗标签抗体 |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-27791.htm
QQ:1749072012