CD86 (B7-2) Monoclonal Antibody (IT2.2), eBioscience

货号：14-0869-82

规格：100 µg

价格：2103

产品类型：流式抗体

品牌：eBioscience

抗原：CD86

物种：人

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：IT2.2

荧光染料：其它

类型:	一抗	同型对照：	IgG2b, kappa
浓度：	0.5 mg/mL	用法：	0.25 µg/test（Flow）

产品详细信息Description: The IT2.2 monoclonal antibody reacts with human CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response.Applications Reported: The IT2.2 antibody has been reported for use in flow cytometric analysis. IT2.2 has also been reported in blocking of CD86 in functional studies. (Please use Functional Grade purified IT2.2, cat. 16-0869, in functional assays.).Applications Tested: The IT2.2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is theligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.仅用于科研。不用于诊断过程。

数据

CD86 (B7-2) Antibody (14-0869-82) in FlowNormal human peripheral blood stained with Anti-Human CD86 (B7-2) Purified followed by Anti-Mouse IgG FITC (Product # 11-4011-85. Appropriate isotype controls were used (open histogram). Cells in the monocyte gate were analyzed.

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文献：

1.OncoimmunologyCD137L-stimulated dendritic cells are more potent than conventional dendritic cells at eliciting cytotoxic T-cell responses."14-0869 was used in Flow cytometry/Cell sorting to develop improved dendritic cell-based immunotherapy using CD137 signalling."AuthorsHarfuddin Z,Kwajah S,Chong Nyi Sim A,Macary PA,Schwarz H2.Experimental and therapeutic medicineAbnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection."Published figure using CD86 (B7-2) monoclonal antibody (Product # 14-0869-82) in Flow Cytometry"3.American journal of respiratory and critical care medicineLung dendritic cell expression of maturation molecules increases with worsening chronic obstructive pulmonary disease."14-0869 was used in Flow cytometry/Cell sorting to determine the expression of maturation molecules by individual DC subsets in relationship to COPD stage."AuthorsFreeman CM,Martinez FJ,Han MK,Ames TM,Chensue SW,Todt JC,Arenberg DA,Meldrum CA,Getty C,McCloskey L,Curtis JL4.BloodSevere loss of invariant NKT cells exhibiting anti-HTLV-1 activity in patients with HTLV-1-associated disorders."14-0869 was used in Flow cytometry/Cell sorting to assess the contribution of iNKT cells to the immune defence against human T-lymphotropic virus type 1 and associated disorders."AuthorsAzakami K,Sato T,Araya N,Utsunomiya A,Kubota R,Suzuki K,Hasegawa D,Izumi T,Fujita H,Aratani S,Fujii R,Yagishita N,Kamijuku H,Kanekura T,Seino K,Nishioka K,Nakajima T,Yamano Y5.American journal of respiratory and critical care medicineLung dendritic cell expression of maturation molecules increases with worsening chronic obstructive pulmonary disease."14-0869 was used in Flow cytometry/Cell sorting to determine the expression of maturation molecules by individual DC subsets in relationship to COPD stage."AuthorsFreeman CM,Martinez FJ,Han MK,Ames TM,Chensue SW,Todt JC,Arenberg DA,Meldrum CA,Getty C,McCloskey L,Curtis JL

技术参数

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