CD80 (B7-1) Monoclonal Antibody (16-10A1), PE-Cyanine7, eBioscience

货号：25-0801-82,25-0801-80

规格：100 µg,25 µg

价格：3163,1267

产品类型：流式抗体

品牌：eBioscience

抗原：CD80

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：16-10A1

荧光染料：PE-Cy7

类型:	一抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), PE-Cyanine7, eBioscience
浓度：	0.2 mg/mL	用法：	1 µg/test（Flow）

产品详细信息Description: The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.Applications Reported: This 16-10A1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 16-10A1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.仅用于科研。不用于诊断过程。

数据

CD80 (B7-1) Antibody (25-0801-82) in FlowStaining of unstimulated (left) or 2-day LPS-stimulated (right) mouse splenocytes with 0.5 µg of Armenian Hamster IgG Isotype Control PE-Cyanine7 (Product # 25-4888-82) (blue histogram) or 0.5 µg of Anti-Mouse CD80 (B7-1) PE-Cyanine7 (purple histogram). Total viable cells were used for analysis.

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参考文献：

1. Cell reportsCancer-Specific Loss of p53 Leads to a Modulation of Myeloid and T Cell Responses."25-0801 was used in Flow cytometry/Cell sorting to show suppressive regulatory T cells accumulate in tumour cells in the absence of p53 indicating a pathway in which cancer cells circumvent immune destruction."AuthorsBlagih J,Zani F,Chakravarty P,Hennequart M,Pilley S,Hobor S,Hock AK,Walton JB,Morton JP,Gronroos E,Mason S,Yang M,McNeish I,Swanton C,Blyth K,Vousden KH2. Frontiers in immunologyFcεRI γ-Chain Negatively Modulates Dectin-1 Responses in Dendritic Cells."Published figure using CD80 (B7-1) monoclonal antibody (Product # 25-0801-82) in Flow Cytometry"3. International journal of molecular sciencescGAS/STING/TBK1/IRF3 Signaling Pathway Activates BMDCs Maturation FollowingMycobacterium bovisInfection."Published figure using CD80 (B7-1) monoclonal antibody (Product # 25-0801-82) in Flow Cytometry"4. Nature communicationsT cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells."Published figure using CD80 (B7-1) monoclonal antibody (Product # 25-0801-82) in Flow Cytometry"5. CellMitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.AuthorsZiegler PK,Bollrath J,Pallangyo CK,Matsutani T,Canli Ö,De Oliveira T,Diamanti MA,Müller N,Gamrekelashvili J,Putoczki T,Horst D,Mankan AK,Öner MG,Müller S,Müller-Höcker J,Kirchner T,Slotta-Huspenina J,Taketo MM,Reinheckel T,Dröse S,Larner AC,Wels WS,Ernst M,Greten TF,Arkan MC,Korn T,Wirth D,Greten FR

技术参数

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