Recombinant Human Interleukin-1RA

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Recombinant Human Interleukin-1RA

货号:PHP080

规格:20 µg

价格:3162

产品类型:细胞因子添加物

品牌:Bio-Rad

产品介绍
Interleukin-1 receptor antagonist (IL-1RA) is a naturally occurring protein which inhibits the activity of IL-1 alpha and IL-1 beta by competitively blocking binding to cell surface receptors for IL-1.In an assay of dose dependent inhibition of IL-1 stimulation of D10S cells, the ED50 was expected to be 20-40 ng/ml.
产品详情
Target SpeciesHuman
Product FormPurified recombinant protein - lyophilized
ReconstitutionReconstitute with 0.2 ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% bovine serum albumin (BSA) is recommended.
PreparationRecombinant protein produced inE. coli
Preservative StabilisersNone present
Carrier FreeYes
Purity>98% by SDS PAGE
Protein Molecular Weight17 kDa (153 amino acids)
Approx. Protein ConcentrationsTotal protein concentration 0.1 mg/ml after reconstitution.
RegulatoryFor research purposes only
GuaranteeGuaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
存储条件
Prior to reconstitution store at -20℃. Following reconstitution store at -20℃.This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
Functional Assays20
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

技术参数

产品应用 elisa,wb,fn

产品类型 重组蛋白

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