Mouse anti Human CD42a antibody, clone FMC-25
货号：MCA594F
规格：0.1MG
价格：4828
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD42a
物种：人
宿主：小鼠
抗体亚型：IgG1
克隆号：FMC-25
荧光染料：FITC
| 产品介绍 | | |
| Mouse anti Human CD42a antibody, clone FMC-25recognizes human CD42a, also known as Platelet glycoprotein IX, Glycoprotein 9 or GP-IX. CD42a is a 177 amino acid, ~20kDa type I single pass transmembrane glycoprotein containing a singleleucine-rich repeat containing N-terminaldomain and a singleleucine-rich repeat containing C-terminaldomain.CD42a is expressed by platelets and megakaryocytes and forms a covalent complex with CD42c (GP-1b-beta), CD42b (GP-1b-alpha) and CD42d (platelet glycoprotein V) to create the platelet surface receptor for von Willebrand factor. Incubation of the intact von Willebrand receptor complex with clone FMC-25 does not appear to inhibit binding of von Willebrand factor to the receptor (Yanet al.2011). Defects in the GP1BB gene encoding human CD42a can lead to the inherited bleeding disorder Bernard-Soulier syndrome (Diz-Küçükkaya 2013), characterized by prolonged bleeding times, thrombocytopenia and the appearance of giant platelets in the circulation (Johnset al.2014).Mouse anti human CD42a antibody, clone FMC-25 has been successfully used as a capture reagent for platelet-autoantibody complexes in the sera of patients presenting thrombocytopenia associated with antiphospholipid syndrome (Godeauet al.1997). |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
| Immunogen | Peripheral blood mononuclear cells. |
| Approx. Protein Concentrations | IgG concentration 0.1 mg/ml |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| FITC | 490 | 525 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at +4℃. DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1.Zola, H.et al.(1984) Monoclonal antibodies against antigens of the human platelet surface: preparation and properties.Pathology. 16 (1): 73-8.2.Berndt, M.C.et al.(1985) Molecular characterization of quinine/quinidine drug-dependent antibody platelet interaction using monoclonal antibodies.Blood. 66 (6): 1292-301.3.Berndt, M.C.et al.(1985) Purification and preliminary characterization of the glycoprotein Ib complex in the human platelet membrane.Eur J Biochem. 151 (3): 637-49.4.Berndt, M.C.et al.(1983) Additional glycoprotein defects in Bernard-Soulier's syndrome: confirmation of genetic basis by parental analysis.Blood. 62 (4): 800-7. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
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