| 产品介绍 | | |
| Rat anti Mouse CD254 antibody, clone IK22/5recognises CD254, otherwise known as Receptor activator of nuclear factor kappa B ligand (RANKL) or TNF-related activation-induced cytokine (TRANCE), a member of the tumour necrosis factor ligand superfamily. CD254 is a cytokine that binds osteoprotegerin or RANK and plays an important role in bone metabolism, where it acts as an osteoclast differentiation and activation factor. Overproduction of CD254 is implicated in a range of degenerative bone diseases.CD254 is also expressed by T helper cells and augments the ability of dendritic cells to stimulate naïve T cell proliferation. It may play a role in regulating interactions between T cells and dendritic cells and in the regulation of T cell-dependent immune responses.Rat anti Mouse CD254 antibody, clone IK22/5 blocks ligand binding. |
| 产品详情 | | |
| Target Species | Mouse |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
| Approx. Protein Concentrations | IgG concentration 0.5mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/20 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Kamijo, S.et al.(2006) Amelioration of bone loss in collagen-induced arthritis by neutralizing anti-RANKL monoclonal antibody.Biochem Biophys Res Commun. 347: 124-32.2.Miyahira, Y.et al.(2003) Cutting edge: a potent adjuvant effect of ligand to receptor activator of NF-kappa B gene for inducing antigen-specific CD8+ T cell response by DNA and viral vector vaccination.J Immunol. 171: 6344-8.3.Miyahira, Y.et al.(2005) Immune responses against a single CD8+-T-cell epitope induced by virus vector vaccination can successfully controlTrypanosoma cruziinfection.Infect Immun. 73: 7356-65.4.Knoop, K.et al. (2009) RANKL is necessary and sufficient to initiate development of antigen-sampling M cells in the intestinal epithelium.J Immunol. 183:5738-47. |
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