Rat anti mouse IgG HEAVY chain:Biotin
货号：MCA424B
规格：0.5 mg
价格：6248
产品类型：二抗
品牌：Bio-Rad
物种：小鼠
宿主：大鼠
抗体亚型：其它
克隆号：COCKTAIL
荧光染料：Biotin
| 产品介绍 | | |
| Rat anti Mouse IgG Heavy Chain antibody cocktailhas been shown to be specific for the gamma heavy chain of mouse immunoglobulin by immunoblotting techniques. It recognizes IgG2a and 2b strongly and IgG1 and IgG3 more weakly. |
| 产品详情 | | |
| Target Species | Mouse |
| Product Form | Purified IgG conjugated to Biotin - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on mouse Ig/Sepharose 4B from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.1%Sodium Azide |
| Immunogen | Purified IgG from BALB/c mice. |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
| Fusion Partners | Spleen cells from immunised LOU/c rats were fused with cells of the IR983F rat myeloma cell line. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1.Bazin, H. (1982) Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line.Prot. Biol. Fluids. Peters Ed: 29th Colloquim (1981). Pergamon Press, Oxford and N.Y., pp 615-618.2.Bazin, H.et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serumJ. Immunol. Methods 71: 9 -16 |
技术参数 产品应用 ELISA
产品类型 Monoclonal Antibody
推荐用于 ELISA
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