Hamster anti Mouse CD27:FITC
货号：MCA4701F
规格：0.1 mg
价格：2550
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：CD27
物种：小鼠
宿主：Hamster
抗体亚型：IgG1, kappa
荧光染料：FITC
| 产品介绍 | | |
| Hamster anti Mouse CD27 antibody, clone LG.3A10recognizes mouse, rat and human CD27, a member of the tumor necrosis factor receptor superfamily. It is expressed in the thymus and spleen, but not in non-lymphoid tissues. CD27 acts as a ligand for CD70, which is expressed in T and B cells, and acts as a co-stimulatory signal in lymphocyte proliferation and immunoglobulin production.CD27 lacks a death domain in its short cytoplasmic tail, but can induce apoptosis by binding with the apoptosis regulatory protein Siva (also known as CD27-binding protein). |
| 产品详情 | | |
| Target Species | Mouse |
| Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
| Preparation | Purified IgG prepared by affinity chromatography |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
| Immunogen | Armenian hamster spleen cells from immunized animals were fused with cells of the murine SP2/0 non-secreting myeloma. |
| Approx. Protein Concentrations | IgG concentration 0.5 mg/ml |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| FITC | 490 | 525 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 同型对照 |
| Description | Product Code | Applications | Pack Size |
| Hamster (Armenian) IgG Negative Control:FITC | MCA2356F | F | 100 Tests |
| 文献 |
| 1.Gravestein, L.A.et al.(1995) Novel mAbs reveal potent co-stimulatory activity of murine CD27.Int Immunol. 7: 551-7. |
技术参数 产品应用 Flow
产品类型 单克隆抗体
推荐用于 Flow
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