| 产品介绍 | | |
| Mouse anti Human CD147 antibody, clone MEM-M6/6recognizes the Ig-like V-type domain of human CD147, otherwise known as basigin, neurothelin or extracellular matrix metalloproteinase inducer (EMMPRIN). CD147 is a 364 amino acid, ~50-55 kDa single pass type I transmembrane glycoprotein and member of the immunoglobulin gene superfamily, containing a single Ig-like C2-type and a single Ig-like V-type domain. CD147 is broadly expressed on peripheral blood cells, endothelial cells, and increased on T cells following activation. CD147 is also strongly expressed on small lung cancer cells and hepatocellular carcinoma.Biological properties of CD147 include the regulation of T cells, integrin functions and cell adhesion, the stimulation of metalloproteinase (MMP) production by fibroblasts, the promotion of astrocyte outgrowths, and roles in tumour progression, neuronal system function and reproduction (Muramatsuet al.2003).There are four distinct isoforms of CD147 described in UniProt entryP35613resulting from alternative splicing or differing promotor usage, the canonical version described here and three alternative truncated isoforms with alterations and deletions to the N-terminal region. The epitope recognized by mouse anti human CD147, clone MEM-M6/6 lies C-terminally to the altered sequences in these isoforms and clone MEM-M6/6 would therefore be expected to bind to all described forms of human CD147.Mouse anti Human CD147, clone MEM-M6/6 inhibits anti-CD3-induced T cell activation, dependent upon antibody concentration (Kochet al.1999). |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Fusion Partners | Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line. |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Immunogen | CD147Rg, consisting of the cDNA coding for the extracellular region of CD147 expressed in CHO cells. |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
| Regulatory | For research purposes only |
| Guarantee | Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date. |
| 存储条件 | | |
| Store at -20oC only.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Western Blotting | √ | 1/100 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1.Koch, C.et al.(1999) T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density.Int Immunol. 11 (5): 777-86.2.Nassif, X.et al.(2015) Cd147 as receptor for pilus-mediated adhesion of meningococci to vascular endothelia.Patent Publication number US20150110806 A13.Bernard SCet al.(2014) PathogenicNeisseria meningitidisutilizes CD147 for vascular colonization.Nat Med. 20 (7): 725-31.4.Douglas, A.D.et al.(2014) Neutralization ofPlasmodium falciparummerozoites by antibodies against PfRH5.J Immunol. 192 (1): 245-58. |
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