| 产品介绍 | | |
| Rat anti Mouse CD279 antibody, clone RMP1-30recognizes mouse CD279, a ~55 kDa cell surface protein, a member of the CD28/CTLA-4 family, otherwise known as Programmed Death-1 (PD-1). CD279 is expressed predominantly on activated T- and B- lymphocytes and on a subset of thymocytes.CD279, an immunoinhibitory receptor, plays a critical role in peripheral tolerance induction and prevention of autoimmune disease (Ansariet al. 2003). Two members of the B7 family, B7-H1 (PD-L1) and B7-DC (PD-L2), have been identified as the ligands for CD279.Rat anti Mouse CD279 antibody, clone RMP1-30 does not block the binding of either B7-H1-Ig or B7-DC-Ig fusion proteins to PD-1 transfected BHK cells. |
| 产品详情 | | |
| Target Species | Mouse |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Fusion Partners | Spleen cells from immunised Sprague Dawley rats were fused with cells of the P3U1 myeloma cell line. |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | PD-1 transferred BHK cells. |
| Approx. Protein Concentrations | Pack Size: 0.1 mgIgG concentration 1 mg/mlPack Size: 0.25 mgIgG concentration 1.0 mg/ml |
| Preservative Stabilisers | Pack Size: 0.1 mg0.09% Sodium Azide (NaN3)Pack Size: 0.25 mg |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| Alexa Fluor®488 | 495 | 519 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/100 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1.Matsumoto, K.et al.(2004) B7-DC regulates asthmatic response by an IFN-gamma-dependent mechanism.J Immunol. 172 (4): 2530-41.2.Bartkowiak, T. (2013) Novel Imaging-Based Techniques Reveal a Role for PD-1/PD-L1 in Tumor Immune Surveillance in the Lung.UT GSBS Dissertations and Theses (Open Access). Paper 354.3.Hu, Z.et al.(2013) Regulatory CD8+ T cells associated with erosion of immune surveillance in persistent virus infection suppressin vitroand have a reversible proliferative defect.J Immunol. 191 (1): 312-22.4.Groh, J.et al.(2018) Teriflunomide attenuates neuroinflammation-related neural damage in mice carrying human PLP1 mutations.J Neuroinflammation. 15 (1): 194. |
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