| 产品介绍 | | |
| Rat anti Mouse ER-MP58 antibody, clone ER-MP58recognizes the murine antigen ER-MP58, which is expressed by all bone marrow-derived M-CSF- and GM-CSF-responsive myeloid blood cell precursors.The expression of ER-MP58 remains at a high level throughout the precursor/monocyte stage and is down-regulated upon maturation into mature macrophages. The ER-MP58 antigen is used to distinguish between early myeloid-committed cells, haematopoietic progenitors cells and as a marker for macrophage development in bone marrow. ER-MP58 is suitable for the identification of myeloid haemopoietic islands in various organs, and for embryonic tissues. |
| 产品详情 | | |
| Target Species | Mouse |
| Product Form | Purified IgG - liquid |
| Reconstitution | Reconstitute with 1 ml distilled water |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Fusion Partners | Cells from immunized rats were fused with cells of the Y3-Ag1.2.3 myeloma cell line. |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Balb/c macrophage precursor cell hybrids |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Preservative Stabilisers | 0.09%Sodium Azide |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 推荐产品 |
| Description | Product Code | Applications | Pack Size |
| Mouse Seroblock FcR | BUF041A | F | 0.5mg |
| 文献 |
| 1.Leenen, P.J.et al.(1990) Murine macrophage precursor characterization. II. Monoclonal antibodies against macrophage precursor antigens.Eur J Immunol. 20 (1): 27-34.2.Henkel, G.W.et al.(1999) Commitment to the monocytic lineage occurs in the absence of the transcription factor PU.1.Blood. 93 (9): 2849-58.3.Nikolic, T.et al.(2003) Developmental stages of myeloid dendritic cells in mouse bone marrow.Int Immunol. 15 (4): 515-24.4.Geutskens, S.B.et al.(2005) Macrophages in the murine pancreas and their involvement in fetal endocrine developmentin vitro.J Leukoc Biol. 78 (4): 845-52.5.Sunderkötter, C.et al.(2004) Subpopulations of mouse blood monocytes differ in maturation stage and inflammatory response.J Immunol. 172: 4410-7. |
QQ:1749072012