| 产品介绍 | | |
| Rat anti Mouse CD197 antibody, clone 4B12recognizes murine C-C chemokine receptor type 7, also known as CD197, Epstein-Barr virus-induced G-protein coupled receptor 1, CCR7 or MIP-3 beta receptor. CD197 is a 354 amino acid ~43 kDa member of the G-protein coupled receptor family. In mice, CD197 is expressed on most naïve T cells, a subset of memory T cells, B cells, mature dendritic cells, NK cells, a subset of thymocytes and CD34+ macrophage progenitor cells.CD197 is a functional receptor for chemokines CCL21 and CCL19. The receptor plays an important role in regulating the trafficking and homing of lymphocytes and dendritic cells to secondary lymphoid organs.Rat anti Mouse CD197 antibody, clone 4B12 does not block the binding of the ligand to CD197. |
| 产品详情 | | |
| Target Species | Mouse |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Carrier Free | Yes |
| Immunogen | CCR7 rat basophilic leukaemia (RBL)-2H3 transfectants. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Fusion Partners | Spleen cells from immunised Lou/c rats were fused with cells of the mouse X63.Ag8.653 myeloma cell line. |
| Preservative Stabilisers | 0.09%Sodium Azide |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/25 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 推荐产品 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C;E;P;WB | 1ml |
| 文献 |
| 1.Ritter, U.et al.(2004) Analysis of the CCR7 expression on murine bone marrow-derived and spleen dendritic cells.J Leukoc Biol. 76 (2): 472-6. |
QQ:1749072012