| 产品介绍 | | |
| Mouse anti Human CD19 antibody, clone LT19recognizes human CD19 also known as T-cell surface antigen Leu-12 or B-lymphocyte surface antigen B4. CD19 is a ~95 kDa type I single pass transmembrane glycoprotein expressed on follicular dendritic cells and B-cells during maturation but is lost on development into plasma cells (de Rieet al.1989).CD19 is the broadest lineage specific marker for B cells and functions as a B-cell co-receptor in conjunction with CD21 (Bradburyet al.1992), CD9, CD81 and CD82 (Horváthet al.1998). CD19 is implicated in the down-regulation of B cell growth and proliferation (Pezzuttoet al.1987). |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG conjugated to Alexa Fluor® 647 - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin |
Immunogen | Normal human peripheral blood lymphocytes. |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
FITC | 490 | 525 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom dateof despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | Neat | 1/10 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude itsuse in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Use 10ul of the suggested working dilution to label 100ul of lysed whole peripheral blood. |
| 数据 |
| Figure A. PE conjugated Mouse anti Human CD22 (MCA2331PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. PE conjugated Mouse anti Human CD22 (MCA2331PE) and FITC conjugated Mouse anti Human CD19 (MCA1940F). All experiments performed on human blood gated on single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer. |
| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests/1ml |
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| 文献 |
| 1. Girbl, T.et al.(2013) CD40-mediated activation of chronic lymphocytic leukemia cells promotes their CD44-dependent adhesion to hyaluronan and restricts CCL21-induced motility.Cancer Res. 73: 561-70.2. Hertzberg, L.et al.(2010) Down syndrome acute lymphoblastic leukemia, a highly heterogeneous disease in which aberrant expression of CRLF2 is associated with mutated JAK2: a report from the International BFM Study Group.Blood. 115: 1006-17.3. Kakko, T.et al.(2011) Inflammatory effects of blood leukocytes: association with vascular function in neuropeptide Y proline 7-genotyped type 2 diabetes patients.Diab Vasc Dis Res. 8: 221-8.4. Dorvignit, D.et al.(2012) Expression and biological characterization of an anti-CD20 biosimilar candidate antibody: a case study.MAbs. 4 (4): 488-96.5. Karlsen, M.et al.(2015) TLR-7 and -9 Stimulation of Peripheral Blood B Cells Indicate Altered TLR Signalling in Primary Sjögren's Syndrome Patients by Increased Secretion of Cytokines.Scand J Immunol. 82 (6): 523-31.6. Clark, L.E.et al.(2018) Vaccine-elicited receptor-binding site antibodies neutralize two New World hemorrhagic fever arenaviruses.Nat Commun. 9 (1): 1884.7. Gu, Y.et al.(2019) Defining the structural basis for human alloantibody binding to human leukocyte antigen allele HLA-A*11:01.Nat Commun. 10 (1): 893.8. Yang, C.et al.(2013) B cells promote tumor progression via STAT3 regulated-angiogenesis.PLoS One. 8 (5): e64159. |
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