Mouse anti Human CD166 antibody, clone 3A6
货号：MCA1926FT,MCA1926F
规格：25 µg,0.1 mg
价格：893,3162
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD166
物种：人
宿主：小鼠
抗体亚型：IgG1
克隆号：3A6
荧光染料：FITC
| 产品介绍 | | |
| Mouse anti Human CD166 antibody, clone 3A6recognizes the 100 kDa adhesion molecule CD166, also known as ALCAM. CD166 is a member of the Ig superfamily and is expressed on activated T-cells, B cells and other cells including thymic epithelial cells, fibroblasts, keratinocytes and neurons. CD6 has been identified as a receptor for ALCAM (Skonieret al.1996).Mouse anti Human CD166 antibody, clone 3A6 is reported to cross-react with CD166 on ovine tissues and provides a useful tool for the identification and characterization of ovine mesenchymal stem cells in conjunction withCD44which is expressed by this cell lineage and the hematopoietic cell markerCD45which is not expressed on mesenchymal stem cells (Sanjurjo-Rodríguezet al.2017). |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Sheep | √ |
| Preparation | - MCA1926F: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- MCA1926FT: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
|
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
| Approx. Protein Concentrations | IgG concentration 0.05mg/ml |
| Fusion Partners | Spleen cells from immunised mice were fused with cells of the P3X63 Ag8 myeloma cell line. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| FITC | 490 | 525 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
| 文献 |
| 1.Yeh, S.P.et al.(2005) Mesenchymal stem cells can be easily isolated from bone marrow of patients with various haematological malignancies but the surface antigens expression may be changed after prolongedex vivoculture.Leukemia. 19: 1505-7.2.Patel, D. D.et al. (1997) CD166 Workshop: Tissue distribution and functional analysis of antibodies reactive for CD166, a ligand for CD6.In Leukocyte Typing IV. Kishimoto, T.et al. eds Garland publishing Inc. New York p. 461-4.3.Tondreau, T.et al.(2008) Gene expression pattern of functional neuronal cells derived from human bone marrow mesenchymal stromal cells.BMC Genomics. 9:166. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
推荐用于 Flow
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