SelecTEV™ Protease，SelecTEV™蛋白酶
货号：30810-1 ,30810-2
规格：1000 U,5000 U
价格：1457,6360
产品类型：蛋白提取/纯化
品牌：Lucigen
| SelecTEV™ Protease is a 27 kD improved form of Tobacco Etch Virus (TEV) protease that has been engineered to be more stable, active, and specific than the native protease. SelecTEV™ Protease is a cysteine protease that recognizes the TEV recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly. This seven-amino-acid sequence is rarely found in proteins, making SelecTEV™ Protease an ideal choice for tag removal of fusion proteins. The optimal temperature for cleavage is 30°C, however the enzyme is active over a wide temperature range (4 – 30°C) and pH (6.0 – 8.5) to accommodate your specific protein. Following digestion, SelecTEV™ Protease is easily removed from the cleavage reaction by affinity chromatography using the polyhistidine tag at the N-terminus of the protease. SelecTEV™ Protease is purified fromE. coliby affinity chromatography using the polyhistidine tag and is 90% pure when visualized on an SDS-PAGE gel. |
| 优点： |
| ▪ High specific activity and specificity;▪ Engineered for stability;▪ 6-Histidine tag for easy removal. |
| 数据： |
| Figure 1. Coomassie-stained SDS-PAGE gel of TEV cleavage reaction. 10 U of SelecTEV Protease was incubated with 30 µg of fusion protein substrate at 30°C for 1 hour in a 100 µL reaction. Marker (lane M), SelecTEV Protease (Lane 1), Uncleaved fusion substrate (Lane 2), Cleaved substrate and SelecTEV Protease. (Lane 3) |
| 组成成分： |
| ▪SelecTEV Protease▪20X SelecTEV Buffer▪100 mM DTT |
技术参数 产品优点 - High specific activity and specificity - Engineered for stability - 6-Histidine tag for easy removal
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