| 产品介绍 | | |
| Mouse anti Human CD81 antibody, clone 1D6recognizes human CD81, a 26 kDa cell surface antigen also known as TAPA-1, and a member of the tetraspanin family. CD81 is widely expressed on human leucocytes and appears to be involved in a variety of cellular leucocytes including activation, proliferation and differentiation.Mouse anti Human CD81 antibody, clone 1D6 is a potent CD81 reagent, induces homotypic adhesion and has powerful anti-proliferative effects. |
| 产品详情 | | |
Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Chimpanzee | √ |
| Sheep | √ |
| Goat | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide |
| Carrier Free | Yes |
| Immunogen | OCI-LY8 cells aggregated by 5A6 (another CD81 antibody) |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Fusion Partners | Spleen cells from immunised Armenian hamsters were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry1 | √ | 1/5 | 1/10 |
| Immunofluorescence | √ | | |
| Immunohistology - Paraffin | √ | | |
| Immunoprecipitation | √ | | |
| Western Blotting | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Use 10ul of the suggested working dilution to label 106 cells in 100ul.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
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| Description | Product Code | Applications | Pack Size |
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| 文献 |
| 1. Schick, M.R. & Levy, S. (1993) The TAPA-1 molecule is associated on the surface of B cells with HLA-DR molecules.J Immunol. 151 (8): 4090-7.2. Levy, S.et al.(1998) CD81 (TAPA-1): a molecule involved in signal transduction and cell adhesion in the immune system.Annu Rev Immunol. 16: 89-109.3. Griebel, P.J.et al.(2007) Cross-reactivity of mAbs to human CD antigens with sheep leukocytes.Vet Immunol Immunopathol. 119: 115-22.4. Welton, J.L.et al(2010) Proteomics analysis of bladder cancer exosomes.Mol Cell Proteomics. 9: 1324-38.5. Davis, W.C.et al.(2007) Use of flow cytometry to identify monoclonal antibodies that recognize conserved epitopes on orthologous leukocyte differentiation antigens in goats, llamas, and rabbits.Vet Immunol Immunopathol. 119: 123-30.6. Flint, M.et al.(1999) Characterization of hepatitis C virus E2 glycoprotein interaction with a putative cellular receptor, CD81.J Virol. 73:6235-44.7. Parthasarathy, V.et al.(2009) Distinct roles for tetraspanins CD9, CD63 and CD81 in the formation of multinucleated giant cells.Immunology. 127: 237-48.8. Rohlena, J.et al.(2009) Endothelial CD81 is a marker of early human atherosclerotic plaques and facilitates monocyte adhesion.Cardiovasc Res. 81: 187-96.9. Ventress, J.K.et al.(2016) Peptides from Tetraspanin CD9 Are Potent Inhibitors of Staphylococcus Aureus Adherence to Keratinocytes.PLoS One. 11 (7): e0160387.10. Mleczko, J.et al.(2018) Extracellular Vesicles from Hypoxic Adipocytes and Obese Subjects Reduce Insulin-Stimulated Glucose Uptake.Mol Nutr Food Res. 62 (5)Feb 20 [Epub ahead of print]. |
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