| 产品介绍 | | |
| Mouse anti Human CD11a antibody, clone 38recognizes the integrin alpha L subunit, also known as CD11a, leukocyte function-associated molecule 1 alpha chain LFA-1 or integrin alpha-L. CD11a is a ~180 kDa glycoprotein expressed by all human leucocytes. CD11a is involved in cell-cell adhesion processes and binds to ICAM-1, 2 and 3.Mouse anti Human CD11a antibody, clone 38 inhibits CD11a mediated adhesion of T cells to ICAM-1 (Stewart et al. 1996) |
| 产品详情 | | |
Target Species | Human |
| Product Form | Purified IgG - liquid |
Preparation | MCA1848: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant MCA1848GA: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
|
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide |
| Carrier Free | Yes |
| Approx. Protein Concentrations | IgG concentration 1 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/50 | 1/100 |
| Immunofluorescence | √ | | |
| Immunohistology - Frozen | √ | | |
| Immunoprecipitation | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Histology Positive Control TissueHuman Tonsil
|
| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG2a Negative Control | MCA929 | Flow | 100 Tests |
| 相关产品 |
| Description | Product Code | Applications | Pack Size |
| Mouse Seroblock FcR | BUF041A | Flow | 0.1 mg |
| Mouse Seroblock FcR | BUF041B | Flow | 0.5 mg |
| 数据 |
| Figure A. RPE conjugated Mouse anti Human CD19 (MCA1940PE) and Alexa Fluor 488 conjugated Mouse IgG1 isotype control (MCA928A488). Figure B. RPE conjugated Mouse anti Human CD19 (MCA1940PE) and Alexa Fluor 488 conjugated Mouse anti Human CD81 (MCA1847A488). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5™ cell analyzer. |
| 文献 |
| 1. Dransfield, I. & Hogg, N. (1989) Regulated expression of Mg2+ binding epitope on leukocyte integrin alpha subunits.EMBO J. 8 (12): 3759-65.2. Berg, L.P.et al.(2002) Functional consequences of noncognate interactions between CD4+ memory T lymphocytes and the endothelium.J Immunol. 168 (7): 3227-34.3. Foster, G.R.et al.(2004) IFN-alpha subtypes differentially affect human T cell motility.J Immunol. 173: 1663-70.4. Evans, B.J.et al.(2006) Shedding of lymphocyte function-associated antigen-1 (LFA-1) in a human inflammatory response.Blood. 107: 3593-9.5. Suchard, S.J.et al.(2010) An LFA-1 (alphaLbeta2) small-molecule antagonist reduces inflammation and joint destruction in murine models of arthritis.J Immunol. 184: 3917-26.6. Porter, J.C. and Hall, A. (2009) Epithelial ICAM-1 and ICAM-2 regulate the egression of human T cells across the bronchial epithelium.FASEB J. 23: 492-502.7. Daehn, I.S. (2010) T-lymphocyte-induced, fas-mediated apoptosis is associated with early keratinocyte differentiation.Exp Dermatol. 19: 372-80.8. Dominical, V.M.et al.(2014) Prominent role of platelets in the formation of circulating neutrophil-red cell heterocellular aggregates in sickle cell anemia.Haematologica. 99 (11): e214-7. |
QQ:1749072012