| 产品介绍 | | |
| Mouse anti Rat CD61 antibody, clone F11recognizes the rat CD61 cell surface antigen, also known as the integrin b3 chain.CD61 is expressed by platelets, megakaryocytes and osteoclasts.Clone F11 has been shown to inhibit thein vitroattachment of osteoclasts to various proteins including vitronectin, fibrinogen and von Willebrand Factor (Sicket al.2009). |
| 产品详情 | | |
| Target Species | Rat |
| Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
| Carrier Free | Yes |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| FITC | 490 | 525 |
| Acknowledgements | This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Helfrich, M.H.et al.(1992) Integrins on rat osteoclasts: characterization of two monoclonal antibodies (F4 and F11) to rat beta 3.J Bone Miner Res. 7 (3): 345-51.2.Shankar, G.et al.(1993) Integrin receptor-mediated mobilisation of intranuclear calcium in rat osteoclasts.J Cell Sci. 105 ( Pt 1): 61-8.3.Sick, E.et al.(2009) Activation of CD47 receptors causes histamine secretion from mast cells.Cell Mol Life Sci. 66: 1271-82.4.Wang, Z.et al.(2015) Microparticle levels after arterial injury andNOtherapy in diabetes.J Surg Res. pii: S0022-4804(15)00867-7 |
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