Mouse anti Human Myeloperoxidase
货号：MCA1757T,MCA1757
规格：20 µg,0.2 mg
价格：1020,3596
产品类型：流式抗体
品牌：Bio-Rad
物种：人
宿主：小鼠
抗体亚型：IgG1
克隆号：2C7
荧光染料：Purified
| 产品介绍 | | |
| Mouse anti Human myeloperoxidase antibody, clone 2C7recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55 kDa) and two light chains (15 kDa), the heavy chains being linked by a disulphide bond.Mouse anti Human Myeloperoxidase antibody, clone 2C7 recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. Mouse anti Human Myeloperoxidase antibody, clone 2C7 also recognizes recombinant MPO in western blots and weakly in ELISA.Mouse anti Human myeloperoxidase antibody, clone 2C7 may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeabilization. Mouse anti Human myeloperoxidase antibody, clone 2C7 did not recognize rat MPO by ELISA (Patryet al.2003). |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Rat | × |
| Dog | √ |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09%Sodium Azide |
| Carrier Free | Yes |
| Immunogen | Human myeloperoxidase |
| Fusion Partners | Spleen cells from immunized mice were fused with cells of the mouse X63 AG8-653 myeloma cell line |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) | STAR117A | F | 0.5mg |
| 文献 |
| 1.Audrain, M.A.et al.(1997) Anti-native and recombinant myeloperoxidase monoclonals and human autoantibodies.Clin Exp Immunol. 107 (1): 127-34. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
推荐用于 Flow
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