产品介绍 | | |
| Mouse anti Growth Cone antibody, clone 2G13recognizes a protein originally termed 2G13P which is localized to growth cones. Subsequent investigation has identified this protein to be 40S ribosomal protein SA, also known as 37 kDa laminin receptor precursor or Laminin receptor 1 (Balouiet al.2004). 40S ribosomal protein SA is a 296 amino acid ~37 kDa membrane, cytoplasmic and nuclear protein required for the assembly and/or stability of the 40S ribosomal subunit.. In vertebrate evolution the molecule has acquired a secondary function as a laminin receptor (UniProt: P50890). In growth cones expression is notable particularly in filopodia and lamellipodia in developing rat CNS and embryonic neurons in culture (Stettleret al.1999).40S ribosomal protein SA interacts with the filamentous actin cytoskeleton and therefore may be involved in growth cone motility (Stettleret al.1999). Mouse anti Growth Cone antibody, clone 2G13 has been used for the detection of growth cones by immunohistochemistry and identification of 40S ribosomal protein SA by western blotting in chicken and rat samples (Balouiet al.2004). |
| 产品详情 | | |
Target Species | Chicken |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Rat | √ |
| Mouse | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Tissue Culture Supernatant - liquid |
Preparation | Tissue Culture Supernatant containing 0.2M Tris/HClpH7.4 and 5-10% foetal calf serum |
| Preservative Stabilisers | 0.09% Sodium Azide |
| Immunogen | Embryonic chick tectal membranes. |
| Fusion Partners | Spleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | X | | |
| Immunohistology - Frozen | √ | | |
| Immunohistology - Paraffin | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Clone 2G13 has also been reported to work in Western Blotting.- ImmunohistologyThis product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
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| 数据 |
| Immunohistochemical staining of growth cones of primary olfactory neurons within the olfactory bulb in the developing rat brain using Mouse anti growth cone antibody (MCA1712). |
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| 文献 |
| 1. Baloui, H.et al.(2004) Cellular prion protein/laminin receptor: distribution in adult central nervous system and characterization of an isoform associated with a subtype of cortical neurons.Eur J Neurosci. 20 (10): 2605-16.2. Espejo, C.et al.(2005) Time-course expression of CNS inflammatory, neurodegenerative tissue repair markers and metallothioneins during experimental autoimmune encephalomyelitis.Neuroscience. 132(4):1135-49.3. Penkowa, M.et al.(2003) Metallothionein-I overexpression alters brain inflammation and stimulates brain repair in transgenic mice with astrocyte-targeted interleukin-6 expression.Glia. 42 (3): 287-306.4. Kim, S.R.et al.(2011) Dopaminergic pathway reconstruction by Akt/Rheb-induced axon regeneration.Ann Neurol. 70: 110-20.5. Nordman, J.C. & Kabbani, N. (2012) An interaction between α7 nicotinic receptors and a G-protein pathway complex regulates neurite growth in neural cells.J Cell Sci. 125 (Pt 22): 5502-13. |
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