Mouse anti Bovine CD8 Beta:RPE
货号：MCA1653PE
规格：100 Tests
价格：4378
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD8 Beta
物种：Bovine
宿主：小鼠
抗体亚型：IgG2a
荧光染料：PE
| 产品介绍 | | |
| Mouse anti Bovine CD4 antibody, clone CC8recognizes bovine CD4, the homolog of human CD4 and immunoprecipitates a ~50 kDa molecule. The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. However, expression on macrophages has not yet been detected. Mouse anti Bovine CD4 antibody, clone CC8 has been reported as being suitable for use on formalin dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques (Eskraet al.1991).A mutation in the bovine CD4 gene resulting in an amino acid substitution at A324 T, located in the D4 domain of the CD4 gene product can occur. This mutation results in lowered binding of Mouse anti Bovine CD4 antibody, clone CC8 to CD4 in Japanese Black (JB) cattle where this mutation has been identified (Kato-Mori,et al.. 2020). CD4 in JB cattle can be identified using clone CACT138A (MCA6081) whose binding to bovine CD4 is unaffected by the A324T mutation (Kato-Mori,et al.. 2020). |
| 产品详情 | | |
| Target Species | Bovine |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Bovine lymphocytes. |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin5% Sucrose |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
| Fusion Partners | Spleen cells from an immunized mouse were fused with cells of the mouse NS1 myeloma cell line. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at +4℃.DO NOT FREEZEThis product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | Neat |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 同型对照 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG2a Negative Control:RPE | MCA929PE | F | 100 Tests |
| 文献 |
| 1.Endsley, J.J.et al.(2004) Characterization of bovine homologues of granulysin and NK-lysin.J Immunol. 173 (4): 2607-14.2.Bensaid, A. & Hadam, M. (1991) Individual antigens of cattle. Bovine CD4 (BoCD4).Vet Immunol Immunopathol. 27 (1-3): 51-4.3.Eskra, L.et al.(1991) Effect of monoclonal antibodies onin vitrofunction of T-cell subsets.Vet Immunol Immunopathol. 27 (1-3): 215-22.4.Howard, C.J.et al.(1991) Summary of workshop findings for leukocyte antigens of cattle.Vet Immunol Immunopathol. 27 (1-3): 21-7. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
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