| 产品介绍 | | |
| Mouse anti Human CD55 antibody, clone 67recognizes the human CD55 cell surface antigen, a GPI linked molecule also known as decay accelerating factor (DAF). CD55 is expressed by a wide range of cell types.CD55 is the complement regulatory protein, decay accelerating factor (DAF) (Lublin and Atkinson 1989). Human CD55 is a ~70 kDa glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells the apparent molecular weight is somewhat larger. It has a substantial content of O-glycans, and also on N-glycan. DAF binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. DAF carries the |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG conjugated to RPE-Alexa Fluor 647 - lyophilized |
| Reconstitution | Reconstitute with 1.0 ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | K562 cells |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin5% Sucrose |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE-Alexa Fluor®647 488nm laser | 496 | 667 |
| Acknowledgements | This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at +4℃.After reconstitution store at +4℃.DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | 1/10 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 同型对照 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:RPE-Alexa Fluor® 647 | MCA928P647 | F | 100 Tests/1ml |
| 文献 |
| 1.Hadam, M.R. (1989) In Leucocyte Typing IV: White Cell Differentiation Antigens.Edited by Knapp, W.et al. Oxford University Press pp 694-697.2.Holmes, C.H.et al.(1990) Preferential expression of the complement regulatory protein decay accelerating factor at the fetomaternal interface during human pregnancy.J Immunol. 144 (8): 3099-105.3.O'Brien, D.P.et al.(2009) Regulation of the Helicobacter pylori cellular receptor decay-accelerating factor.J Biol Chem. 283: 23922-30.4.Yang, P.et al.(2009) Expression and modulation of RPE cell membrane complement regulatory proteins.Invest Ophthalmol Vis Sci. 50: 3473-81. |
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