Mouse anti Human mast cell tryptase, clone AA1
货号：MCA1438,MCA1438T
规格：0.2 mg,20 µg
价格：893,3162
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：Mast Cell Tryptase
物种：人
宿主：小鼠
抗体亚型：IgG1
克隆号：AA1
荧光染料：Purified
| 产品介绍 | | |
| Mouse anti Human mast cell tryptase, clone AA1recognizes human mast cell tryptase, bothalpha and betaisoforms. Mouse anti Mast cell tryptase, clone AA1 is an excellent marker for mast cells, and does not bind to any other cell type in immunohistology (Wallset al.1990).Tryptases are the products of a number ofgenesand form the major neutral protease present in mast cells secreted in response to infection and injury. Mast cell tryptase has an important role in the pathology of inflammatory diseases, especially asthma through bronchoconstriction (Zhang and Timmerman 1997). |
| 产品详情 | | |
Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Dog | √ |
| Monkey | √ |
| Cat | √ |
| Rat | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide |
| Carrier Free | Yes |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Fusion Partners | Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | |
| Western Blotting | √ | | |
| Immunofluorescence | √ | | |
| Immunohistology - Frozen | √ | | |
| Immunohistology - Paraffin1 | √ | | 1/10000 |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Histology Positive Control Tissue
- Tonsil
|
| 文献 |
| 1. Liu, J.et al.(2009) Genetic deficiency and pharmacological stabilization of mast cells reduce diet-induced obesity and diabetes in mice.Nat Med. 15: 940-5.2.Asano-Kato, N.et al.(2005) Tryptase increases proliferative activity of human conjunctival fibroblasts through protease-activated receptor-2.Invest Ophthalmol Vis Sci. 46: 4622-6.3. Facoetti, A.et al.(2006) Histochemical study of cardiac mast cells degranulation and collagen deposition: interaction with the cathecolaminergic system in the rat.Eur J Histochem. 50: 133-40.4. Dichlberger, A.et al.(2011) Lipid body formation during maturation of human mast cells.J Lipid Res. 52: 2198-208.5. Kawarai, S.et al.(2010) Cultivation and characterization of canine skin-derived mast cells.J Vet Med Sci. 72 (2): 131-40.6. Kazama, I.et al.(2015) Mast cell involvement in the progression of peritoneal fibrosis in rats with chronic renal failure.Nephrology (Carlton). 20 (9): 609-16.7. Kato, Y.et al.(2016) Cutaneous mastocytosis with a mutation in the juxtamembrane domain of c-kit in a young laboratory beagle dog.J Toxicol Pathol. 29 (1): 49-52.8. Luo, J.et al.(2016) An indispensable role of CPT-1a to survive cancer cells during energy stress through rewiring cancer metabolism.Tumour Biol. Oct 13 [Epub ahead of print].9. Perbellini, O.et al.(2011) Primary role of multiparametric flow cytometry in the diagnostic work-up of indolent clonal mast cell disorders.Cytometry B Clin Cytom. 80 (6): 362-8.10. Baba, A.et al.(2017) Less contribution of mast cells to the progression of renal fibrosis in Rat kidneys with chronic renal failure.Nephrology (Carlton). 22 (2): 159-67. |
技术参数 产品应用 IHC;ELISA;IF;WB
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-33087.htm
QQ:1749072012